Surface plasmon resonance: An innovative method for studying water permeability of plant cuticles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F21%3AA2202EN0" target="_blank" >RIV/61988987:17310/21:A2202EN0 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60076658:12310/21:43903636 RIV/60077344:_____/21:00548345 RIV/61989100:27360/21:10247633 RIV/61989100:27710/21:10247633

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0168945221001746" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0168945221001746</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plantsci.2021.110978" target="_blank" >10.1016/j.plantsci.2021.110978</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Surface plasmon resonance: An innovative method for studying water permeability of plant cuticles

Popis výsledku v původním jazyce

The cuticle forms an effective barrier protecting plants from water loss. Its permeability to water and other compounds significantly differs between species, types of cuticle (stomatous, astomatous), and can be affected by a wide variety of ambient conditions. Enzymatic isolation of the leaf cuticle allows obtaining intact cuticles for permeability measurements. However, the most available gravimetric method, which is used for the assessment of water permeability of isolated cuticles, requires a relatively large area of the cuticle and does not allow the determination of membrane heterogeneity. We propose a new method for the determination of water permeance based on an on-line detection of water flux from a liquid phase to the atmosphere through isolated leaf cuticles in semi-flow chambers. This approach is new in using the phenomenon of surface plasmon resonance for the detection of the liquid phase refractive index affected by water vapor. Isolated cuticles of the leaves of Ficus elastica and an artificial polyethersulfone membrane were used for method evaluation. The composition of cuticular wax and its influence on cuticular permeability was also studied. It has been confirmed that the application of the surface plasmon resonance principle can be used for the assessment of leaf cuticle water permeability and heterogeneity.

Název v anglickém jazyce

Surface plasmon resonance: An innovative method for studying water permeability of plant cuticles

Popis výsledku anglicky

The cuticle forms an effective barrier protecting plants from water loss. Its permeability to water and other compounds significantly differs between species, types of cuticle (stomatous, astomatous), and can be affected by a wide variety of ambient conditions. Enzymatic isolation of the leaf cuticle allows obtaining intact cuticles for permeability measurements. However, the most available gravimetric method, which is used for the assessment of water permeability of isolated cuticles, requires a relatively large area of the cuticle and does not allow the determination of membrane heterogeneity. We propose a new method for the determination of water permeance based on an on-line detection of water flux from a liquid phase to the atmosphere through isolated leaf cuticles in semi-flow chambers. This approach is new in using the phenomenon of surface plasmon resonance for the detection of the liquid phase refractive index affected by water vapor. Isolated cuticles of the leaves of Ficus elastica and an artificial polyethersulfone membrane were used for method evaluation. The composition of cuticular wax and its influence on cuticular permeability was also studied. It has been confirmed that the application of the surface plasmon resonance principle can be used for the assessment of leaf cuticle water permeability and heterogeneity.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Science

ISSN

0168-9452

e-ISSN

—

Svazek periodika

310

Číslo periodika v rámci svazku

září 2021

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

9

Strana od-do

1-9

Kód UT WoS článku

000679382700003

EID výsledku v databázi Scopus

2-s2.0-85108844903