A novel approach for measuring membrane permeability for organic compounds via surface plasmon resonance detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F23%3AA2402J6Q" target="_blank" >RIV/61988987:17310/23:A2402J6Q - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989100:27360/23:10250864 RIV/61989100:27710/23:10250864

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S004565352203658X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S004565352203658X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chemosphere.2022.137165" target="_blank" >10.1016/j.chemosphere.2022.137165</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A novel approach for measuring membrane permeability for organic compounds via surface plasmon resonance detection

Popis výsledku v původním jazyce

Well-known methods for measuring permeability of membranes include static or flow diffusion chambers. When studying the effects of organic compounds on plants, the use of such model systems allows to investigate xenobiotic behavior at the cuticular barrier level and obtain an understanding of the initial penetration processes of these substances into plant leaves. However, the use of diffusion chambers has disadvantages, including being time-consuming, requiring sampling, or a sufficiently large membrane area, which cannot be obtained from all types of plants. Therefore, we propose a new method based on surface plasmon resonance imaging (SPRi) to enable rapid membrane permeability evaluation. This study presents the methodology for measuring permeability of isolated cuticles for organic compounds via surface plasmon resonance detection, where the selected model analyte was the widely used pesticide metazachlor. Experiments were performed on the cuticles of Ficus elastica, Citrus pyriformis, and an artificial PES membrane, which is used in passive samplers for the detection of xenobiotics in water and soils. The average permeability for metazachlor was 5.23 x 10-14 m2 s- 1 for C. pyriformis, 1.34 x 10-13 m2 s- 1 for F. elastica, and 7.74 x 10-12 m2 s- 1 for the PES membrane. We confirmed that the combination of a flow-through diffusion cell and real-time optical detection of transposed molecules represents a promising method for determining the permeability of membranes to xenobiotics occurring in the environment. This is necessary for determining a pesticide dosage in agriculture, selecting suitable membranes

Název v anglickém jazyce

A novel approach for measuring membrane permeability for organic compounds via surface plasmon resonance detection

Popis výsledku anglicky

Well-known methods for measuring permeability of membranes include static or flow diffusion chambers. When studying the effects of organic compounds on plants, the use of such model systems allows to investigate xenobiotic behavior at the cuticular barrier level and obtain an understanding of the initial penetration processes of these substances into plant leaves. However, the use of diffusion chambers has disadvantages, including being time-consuming, requiring sampling, or a sufficiently large membrane area, which cannot be obtained from all types of plants. Therefore, we propose a new method based on surface plasmon resonance imaging (SPRi) to enable rapid membrane permeability evaluation. This study presents the methodology for measuring permeability of isolated cuticles for organic compounds via surface plasmon resonance detection, where the selected model analyte was the widely used pesticide metazachlor. Experiments were performed on the cuticles of Ficus elastica, Citrus pyriformis, and an artificial PES membrane, which is used in passive samplers for the detection of xenobiotics in water and soils. The average permeability for metazachlor was 5.23 x 10-14 m2 s- 1 for C. pyriformis, 1.34 x 10-13 m2 s- 1 for F. elastica, and 7.74 x 10-12 m2 s- 1 for the PES membrane. We confirmed that the combination of a flow-through diffusion cell and real-time optical detection of transposed molecules represents a promising method for determining the permeability of membranes to xenobiotics occurring in the environment. This is necessary for determining a pesticide dosage in agriculture, selecting suitable membranes

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

<a href="/cs/project/EF17_049%2F0008419" target="_blank" >EF17_049/0008419: Podpora mezisektorové spolupráce v oblasti snižování polutantů v životním prostředí a využití odpadů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemosphere

ISSN

0045-6535

e-ISSN

1879-1298

Svazek periodika

—

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

1-9

Kód UT WoS článku

000903544400005

EID výsledku v databázi Scopus

2-s2.0-85142201594