Oect multielectrode array for real-time cell culture monitoring

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F19%3APU136047" target="_blank" >RIV/00216305:26310/19:PU136047 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/19:00523258

Výsledek na webu

<a href="https://www.scopus.com/record/display.uri?eid=2-s2.0-85062984835&origin=resultslist&sort=plf-f&src=s&sid=44723808f725a148aba6e39e5457150f&sot=autdocs&sdt=autdocs&sl=17&s=AU-ID%288378482500%29&relpos=8&citeCnt=0&searchTerm=" target="_blank" >https://www.scopus.com/record/display.uri?eid=2-s2.0-85062984835&origin=resultslist&sort=plf-f&src=s&sid=44723808f725a148aba6e39e5457150f&sot=autdocs&sdt=autdocs&sl=17&s=AU-ID%288378482500%29&relpos=8&citeCnt=0&searchTerm=</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Oect multielectrode array for real-time cell culture monitoring

Popis výsledku v původním jazyce

The significant cost of newly developed pharmacological products is due to the toxicity examination performed on animals in pre-clinical trials. Our ultimate goal is to develop a biosensor array to determine acute cardiotoxicity. It capitalizes on the fact that heart muscle cells (cardiomyocytes) show beating in vitro. Based on the beating characteristics their pathophysiological status can be deducted. In addition to microscopic imaging, the method of monitoring the action and field potential of synchronously beating cardiomyocyte syncytia on multielectrode arrays (MEA) appears to be a suitable method for the investigation of the condition of cardiac cells. For this purpose, MEA on the basis of organic electrochemical transistors (OECTs) has been developed. The organic polymer PEDOT:PSS was utilized to meet the strict surface biocompatibility demand for a surface in contact with media and living cardiac cells. The MEA consisted of a microplate of a 12x8 chimney-well array with transistors on the bottom for cell cultivation. For the fabrication (a screen printing method) primarily a contact field and an organic polymer was used. The device was tested in a simulation mode for the response of the drain current to the voltage pulses applied to the gate electrode up to 100 mu V. The response to such gate voltage renders the device sensitive enough to detect the electrical signal of beating cardiomyocytes. Further development is directed towards higher time resolution by means of miniaturization of the transistors' channel.

Název v anglickém jazyce

Oect multielectrode array for real-time cell culture monitoring

Popis výsledku anglicky

The significant cost of newly developed pharmacological products is due to the toxicity examination performed on animals in pre-clinical trials. Our ultimate goal is to develop a biosensor array to determine acute cardiotoxicity. It capitalizes on the fact that heart muscle cells (cardiomyocytes) show beating in vitro. Based on the beating characteristics their pathophysiological status can be deducted. In addition to microscopic imaging, the method of monitoring the action and field potential of synchronously beating cardiomyocyte syncytia on multielectrode arrays (MEA) appears to be a suitable method for the investigation of the condition of cardiac cells. For this purpose, MEA on the basis of organic electrochemical transistors (OECTs) has been developed. The organic polymer PEDOT:PSS was utilized to meet the strict surface biocompatibility demand for a surface in contact with media and living cardiac cells. The MEA consisted of a microplate of a 12x8 chimney-well array with transistors on the bottom for cell cultivation. For the fabrication (a screen printing method) primarily a contact field and an organic polymer was used. The device was tested in a simulation mode for the response of the drain current to the voltage pulses applied to the gate electrode up to 100 mu V. The response to such gate voltage renders the device sensitive enough to detect the electrical signal of beating cardiomyocytes. Further development is directed towards higher time resolution by means of miniaturization of the transistors' channel.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Projekt

<a href="/cs/project/GA17-24707S" target="_blank" >GA17-24707S: Nové přírodou inspirované organické polovodiče pro bioelektroniku</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON 2018 - Conference Proceedings

ISBN

978-80-872-9489-5

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

336-341

Název nakladatele

TANGER Ltd.

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

17. 10. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000513131900058