Toxicity evaluation of monodisperse PEGylated magnetic nanoparticles for nanomedicine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F19%3APU136161" target="_blank" >RIV/00216305:26310/19:PU136161 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389013:_____/19:00506160

Výsledek na webu

<a href="https://www.tandfonline.com/doi/abs/10.1080/17435390.2018.1555624?scroll=top&needAccess=true&journalCode=inan20" target="_blank" >https://www.tandfonline.com/doi/abs/10.1080/17435390.2018.1555624?scroll=top&needAccess=true&journalCode=inan20</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/17435390.2018.1555624" target="_blank" >10.1080/17435390.2018.1555624</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Toxicity evaluation of monodisperse PEGylated magnetic nanoparticles for nanomedicine

Popis výsledku v původním jazyce

Innovative nanotechnology aims to develop particles that are small, monodisperse, smart, and do not cause unintentional side effects. Uniform magnetic Fe3O4 nanoparticles (12nm in size) were prepared by thermal decomposition of iron(III) oleate. To make them colloidally stable and dispersible in water and cell culture medium, they were modified with phosphonic acid- (PA) and hydroxamic acid (HA)-terminated poly(ethylene glycol) yielding PA-PEG@Fe3O4 and HA-PEG@Fe3O4 nanoparticles; conventional gamma-Fe2O3 particles were prepared as a control. Advanced techniques were used to evaluate the properties and safety of the particles. Completeness of the nanoparticle coating was tested by real-time polymerase chain reaction. Interaction of the particles with primary human peripheral blood cells, cellular uptake, cytotoxicity, and immunotoxicity were also investigated. Amount of internalized iron in peripheral blood mononuclear cells was 72, 38, and 25pg Fe/cell for HA-PEG@Fe3O4, gamma-Fe2O3, and PA-PEG@Fe3O4, respectively. Nanoparticles were localized within the cytoplasm and in the extracellular space. No cytotoxic effect of both PEGylated nanoparticles was observed (0.12-75 mu g/cm(2)) after 24 and 72-h incubation. Moreover, no suppressive effect was found on the proliferative activity of T-lymphocytes and T-dependent B-cell response, phagocytic activity of monocytes and granulocytes, and respiratory burst of phagocytes. Similarly, no cytotoxic effect of gamma-Fe2O3 particles was observed. However, they suppressed the proliferative activity of T-lymphocytes (75 mu g/cm(2), 72h) and also decreased the phagocytic activity of monocytes (15 mu g/cm(2), 24h; 3-75 mu g/cm(2), 72h). We thus show that newly developed particles have great potential especially in cancer diagnostics and therapy

Název v anglickém jazyce

Toxicity evaluation of monodisperse PEGylated magnetic nanoparticles for nanomedicine

Popis výsledku anglicky

Innovative nanotechnology aims to develop particles that are small, monodisperse, smart, and do not cause unintentional side effects. Uniform magnetic Fe3O4 nanoparticles (12nm in size) were prepared by thermal decomposition of iron(III) oleate. To make them colloidally stable and dispersible in water and cell culture medium, they were modified with phosphonic acid- (PA) and hydroxamic acid (HA)-terminated poly(ethylene glycol) yielding PA-PEG@Fe3O4 and HA-PEG@Fe3O4 nanoparticles; conventional gamma-Fe2O3 particles were prepared as a control. Advanced techniques were used to evaluate the properties and safety of the particles. Completeness of the nanoparticle coating was tested by real-time polymerase chain reaction. Interaction of the particles with primary human peripheral blood cells, cellular uptake, cytotoxicity, and immunotoxicity were also investigated. Amount of internalized iron in peripheral blood mononuclear cells was 72, 38, and 25pg Fe/cell for HA-PEG@Fe3O4, gamma-Fe2O3, and PA-PEG@Fe3O4, respectively. Nanoparticles were localized within the cytoplasm and in the extracellular space. No cytotoxic effect of both PEGylated nanoparticles was observed (0.12-75 mu g/cm(2)) after 24 and 72-h incubation. Moreover, no suppressive effect was found on the proliferative activity of T-lymphocytes and T-dependent B-cell response, phagocytic activity of monocytes and granulocytes, and respiratory burst of phagocytes. Similarly, no cytotoxic effect of gamma-Fe2O3 particles was observed. However, they suppressed the proliferative activity of T-lymphocytes (75 mu g/cm(2), 72h) and also decreased the phagocytic activity of monocytes (15 mu g/cm(2), 24h; 3-75 mu g/cm(2), 72h). We thus show that newly developed particles have great potential especially in cancer diagnostics and therapy

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

<a href="/cs/project/LO1211" target="_blank" >LO1211: Centrum materiálového výzkumu na FCH VUT v Brně - udržitelnost a rozvoj</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

NANOTOXICOLOGY

ISSN

1743-5390

e-ISSN

1743-5404

Svazek periodika

13

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

17

Strana od-do

510-526

Kód UT WoS článku

000477976100006

EID výsledku v databázi Scopus

2-s2.0-85060941650