Functionalized porous silica&maghemite core-shell nanoparticles for applications in medicine: design, synthesis and immunotoxicity
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F16%3A00458566" target="_blank" >RIV/61389013:_____/16:00458566 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Functionalized porous silica&maghemite core-shell nanoparticles for applications in medicine: design, synthesis and immunotoxicity
Popis výsledku v původním jazyce
To determine cytotoxicity and effect of silica-coated magnetic nanoparticles (MNPs) on immune response, in particular lymphocyte proliferative activity, phagocytic activity, and leukocyte respiratory burst and in vitro production of interleukin-6 (IL-6) and 8 (IL-8), interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), and granulocyte macrophage colony stimulating factor (GM-CSF). Maghemite was prepared by coprecipitation of iron salts with ammonia, oxidation with NaOCl and modified by tetramethyl orthosilicate and aminosilanes. Particles were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared (FTIR), and X-ray photoelectron spectroscopy (XPS). Cytotoxicity and lymphocyte proliferative activity were assessed using [H-3]-thymidine incorporation into DNA of proliferating human peripheral blood cells. Phagocytic activity and leukocyte respiratory burst were measured by flow cytometry; cytokine levels in cell supernatants were determined by ELISA. The cytotoxicity and in vitro immunotoxicity were found to be minimal in the newly developed porous core-shell gamma-Fe2O3&SiO2-NH2 magnetic nanoparticles.
Název v anglickém jazyce
Functionalized porous silica&maghemite core-shell nanoparticles for applications in medicine: design, synthesis and immunotoxicity
Popis výsledku anglicky
To determine cytotoxicity and effect of silica-coated magnetic nanoparticles (MNPs) on immune response, in particular lymphocyte proliferative activity, phagocytic activity, and leukocyte respiratory burst and in vitro production of interleukin-6 (IL-6) and 8 (IL-8), interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), and granulocyte macrophage colony stimulating factor (GM-CSF). Maghemite was prepared by coprecipitation of iron salts with ammonia, oxidation with NaOCl and modified by tetramethyl orthosilicate and aminosilanes. Particles were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier-transform infrared (FTIR), and X-ray photoelectron spectroscopy (XPS). Cytotoxicity and lymphocyte proliferative activity were assessed using [H-3]-thymidine incorporation into DNA of proliferating human peripheral blood cells. Phagocytic activity and leukocyte respiratory burst were measured by flow cytometry; cytokine levels in cell supernatants were determined by ELISA. The cytotoxicity and in vitro immunotoxicity were found to be minimal in the newly developed porous core-shell gamma-Fe2O3&SiO2-NH2 magnetic nanoparticles.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CD - Makromolekulární chemie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GC16-01128J" target="_blank" >GC16-01128J: Magnetické nanočástice s antioxidačním účinkem na bázi přírodních antioxidantů: Interakce nanočástic s buňkami</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Croatian Medical Journal
ISSN
0353-9504
e-ISSN
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Svazek periodika
57
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
HR - Chorvatská republika
Počet stran výsledku
14
Strana od-do
165-178
Kód UT WoS článku
000377053400009
EID výsledku v databázi Scopus
2-s2.0-84971543394