Preparation and characterisation of organic UV filters based on combined PHB/liposomes with natural phenolic compounds

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F20%3APU138037" target="_blank" >RIV/00216305:26310/20:PU138037 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2590155920300081" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2590155920300081</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.btecx.2020.100021" target="_blank" >10.1016/j.btecx.2020.100021</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Preparation and characterisation of organic UV filters based on combined PHB/liposomes with natural phenolic compounds

Popis výsledku v původním jazyce

In this study preparation and characterization of new UV-protecting systems based on liposomes/polyhydroxybutyrate (PHB) with encapsulated coffee extracts are presented. Green and roasted coffee extracts with high phenolics content, high antioxidant activity and sun protection factor (SPF) value 40–50 were used as model organic UV filters and encapsulated into liposomes and PHB-liposomes. Particle size and colloid stability was observed by dynamic light scattering and zeta-potential. Toxicity of particles was tested by MTT and LDH assay on HaCaT cell line. All prepared samples showed moderate or high encapsulation efficiency. Addition of PHB up to 50 % of lecithin led to increased size and stability. As optimal addition of 20 % PHB into liposome particles was found leading to optimum size and processing of particles, to high UV-protective effect as well as to increased colloid stability and SPF value during long-term storage. Significant differences in cell viability were found in cytotoxicity studies after exposure of keratinocytes to liposomes with different PHB content. Newly fabricated PHB-liposome particles with coffee extract were not found as toxic for HaCaT cells and in LDH test up to 12 %. These particles can act as active carriers for organic sunscreen components in combination with UV-protective effect of PHB.

Název v anglickém jazyce

Preparation and characterisation of organic UV filters based on combined PHB/liposomes with natural phenolic compounds

Popis výsledku anglicky

In this study preparation and characterization of new UV-protecting systems based on liposomes/polyhydroxybutyrate (PHB) with encapsulated coffee extracts are presented. Green and roasted coffee extracts with high phenolics content, high antioxidant activity and sun protection factor (SPF) value 40–50 were used as model organic UV filters and encapsulated into liposomes and PHB-liposomes. Particle size and colloid stability was observed by dynamic light scattering and zeta-potential. Toxicity of particles was tested by MTT and LDH assay on HaCaT cell line. All prepared samples showed moderate or high encapsulation efficiency. Addition of PHB up to 50 % of lecithin led to increased size and stability. As optimal addition of 20 % PHB into liposome particles was found leading to optimum size and processing of particles, to high UV-protective effect as well as to increased colloid stability and SPF value during long-term storage. Significant differences in cell viability were found in cytotoxicity studies after exposure of keratinocytes to liposomes with different PHB content. Newly fabricated PHB-liposome particles with coffee extract were not found as toxic for HaCaT cells and in LDH test up to 12 %. These particles can act as active carriers for organic sunscreen components in combination with UV-protective effect of PHB.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

<a href="/cs/project/LO1211" target="_blank" >LO1211: Centrum materiálového výzkumu na FCH VUT v Brně - udržitelnost a rozvoj</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biotechnology

ISSN

0168-1656

e-ISSN

1873-4863

Svazek periodika

324

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

1-10

Kód UT WoS článku

000710701700006

EID výsledku v databázi Scopus

2-s2.0-85085546099