Newly designed primers targeting limonene synthase
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F21%3APU139903" target="_blank" >RIV/00216305:26310/21:PU139903 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.tandfonline.com/doi/full/10.1080/13102818.2020.1871545?fbclid=IwAR0gLYRgommM-eySZC1qddUErA0VWyAlsj5S0gYm3QG8A0HLbrOJyJyu624" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/13102818.2020.1871545?fbclid=IwAR0gLYRgommM-eySZC1qddUErA0VWyAlsj5S0gYm3QG8A0HLbrOJyJyu624</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Newly designed primers targeting limonene synthase
Popis výsledku v původním jazyce
Cosmetic products with ingredients of plant origin promise beneficial effects on the customer health. Those may contain allergens that belong to the group of 24 fragrance allergens that can negatively affect the health of sensitive individuals. One of these allergens is d-limonene present for example in citrus fruits. The presence of plant DNA in the cosmetic product can be detected using techniques of molecular biotechnology, even if it is present in very small quantity. New primers Lim2 F/R, targeting limonene synthase, were designed to confirm the presence of the citrus DNA in a leave-on cosmetic product. The DNA of Citrus limon was isolated from the leave-on cosmetic product and the specific sequence was amplified using real-time PCR. The presence of amplicons of specific size was confirmed using agarose electrophoresis.
Název v anglickém jazyce
Newly designed primers targeting limonene synthase
Popis výsledku anglicky
Cosmetic products with ingredients of plant origin promise beneficial effects on the customer health. Those may contain allergens that belong to the group of 24 fragrance allergens that can negatively affect the health of sensitive individuals. One of these allergens is d-limonene present for example in citrus fruits. The presence of plant DNA in the cosmetic product can be detected using techniques of molecular biotechnology, even if it is present in very small quantity. New primers Lim2 F/R, targeting limonene synthase, were designed to confirm the presence of the citrus DNA in a leave-on cosmetic product. The DNA of Citrus limon was isolated from the leave-on cosmetic product and the specific sequence was amplified using real-time PCR. The presence of amplicons of specific size was confirmed using agarose electrophoresis.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů