AUTOMATED ZINC PROTEINS EXTRACTION FROM PROSTATIC CANCER CELLS USING MAGNETIC PARTICLES
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F12%3APU126801" target="_blank" >RIV/00216305:26620/12:PU126801 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
AUTOMATED ZINC PROTEINS EXTRACTION FROM PROSTATIC CANCER CELLS USING MAGNETIC PARTICLES
Popis výsledku v původním jazyce
Prostate cancer is a huge problem in modern world, mainly in the developed countries. It is important to diagnose this disease as soon as possible due to its high mortality. Prostate tissue has a unique ability to accumulate zinc ions. However cancer prostate tissue loses this ability. It is therefore possible to help the diagnosis of the prostate cancer via the isolation of zinc proteins contained in prostatic tissue. This isolation can be carried out using magnetic particles modified with anti-Zn-II-antibodies. Our objective was to create a procedure for isolation of zinc proteins from prostatic cells using magnetic particles and automated pipetting station. Cell lines PNT1A and LNCaP were used for experiments. Cell line PNT1A represents healthy prostate tissue, line LNCaP represent cancer tissue. There was decreasing level (10%) of Zn-II ions observed in the cancer prostatic cell lines for the 25 and 50 mu M concentrations of zinc.
Název v anglickém jazyce
AUTOMATED ZINC PROTEINS EXTRACTION FROM PROSTATIC CANCER CELLS USING MAGNETIC PARTICLES
Popis výsledku anglicky
Prostate cancer is a huge problem in modern world, mainly in the developed countries. It is important to diagnose this disease as soon as possible due to its high mortality. Prostate tissue has a unique ability to accumulate zinc ions. However cancer prostate tissue loses this ability. It is therefore possible to help the diagnosis of the prostate cancer via the isolation of zinc proteins contained in prostatic tissue. This isolation can be carried out using magnetic particles modified with anti-Zn-II-antibodies. Our objective was to create a procedure for isolation of zinc proteins from prostatic cells using magnetic particles and automated pipetting station. Cell lines PNT1A and LNCaP were used for experiments. Cell line PNT1A represents healthy prostate tissue, line LNCaP represent cancer tissue. There was decreasing level (10%) of Zn-II ions observed in the cancer prostatic cell lines for the 25 and 50 mu M concentrations of zinc.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
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OECD FORD obor
30204 - Oncology
Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
MENDELNET 2012
ISBN
978-80-7375-836-3
ISSN
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e-ISSN
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Počet stran výsledku
9
Strana od-do
1174-1182
Název nakladatele
Neuveden
Místo vydání
Neuveden
Místo konání akce
Mendel Univ, Fac Agron, Brno
Datum konání akce
21. 11. 2012
Typ akce podle státní příslušnosti
CST - Celostátní akce
Kód UT WoS článku
000366461200137