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Development of a magnetic electrochemical bar code array for point mutation detection in the H5N1 neuraminidase gene

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F13%3APU104593" target="_blank" >RIV/00216305:26620/13:PU104593 - isvavai.cz</a>

  • Nalezeny alternativní kódy

    RIV/62156489:43210/13:00213116 RIV/62157124:16370/13:43871833 RIV/00216224:14310/13:00107096

  • Výsledek na webu

    <a href="https://www.mdpi.com/1999-4915/5/7/1719" target="_blank" >https://www.mdpi.com/1999-4915/5/7/1719</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3390/v5071719" target="_blank" >10.3390/v5071719</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Development of a magnetic electrochemical bar code array for point mutation detection in the H5N1 neuraminidase gene

  • Popis výsledku v původním jazyce

    Since its first official detection in the Guangdong province of China in 1996, the highly pathogenic avian influenza virus of H5N1 subtype (HPAI H5N1) has reportedly been the cause of outbreaks in birds in more than 60 countries, 24 of which were European. The main issue is still to develop effective antiviral drugs. In this case, single point mutation in the neuraminidase gene, which causes resistance to antiviral drug and is, therefore, subjected to many studies including ours, was observed. In this study, we developed magnetic electrochemical bar code array for detection of single point mutations (mismatches in up to four nucleotides) in H5N1 neuraminidase gene. Paramagnetic particles Dynabeads with covalently bound oligo (dT)25 were used as a tool for isolation of complementary H5N1 chains (H5N1 Zhejin, China and Aichi). For detection of H5N1 chains, oligonucleotide chains of lengths of 12 (+5 adenine) or 28 (+5 adenine) bp labeled with quantum dots (CdS, ZnS and/or PbS) were used. Individual probes hybridized to target molecules specifically with efficiency higher than 60%. The obtained signals identified mutations present in the sequence. Suggested experimental procedure allows obtaining further information from the redox signals of nucleic acids. Moreover, the used biosensor exhibits sequence specificity and low limits of detection of subnanogram quantities of target nucleic acids

  • Název v anglickém jazyce

    Development of a magnetic electrochemical bar code array for point mutation detection in the H5N1 neuraminidase gene

  • Popis výsledku anglicky

    Since its first official detection in the Guangdong province of China in 1996, the highly pathogenic avian influenza virus of H5N1 subtype (HPAI H5N1) has reportedly been the cause of outbreaks in birds in more than 60 countries, 24 of which were European. The main issue is still to develop effective antiviral drugs. In this case, single point mutation in the neuraminidase gene, which causes resistance to antiviral drug and is, therefore, subjected to many studies including ours, was observed. In this study, we developed magnetic electrochemical bar code array for detection of single point mutations (mismatches in up to four nucleotides) in H5N1 neuraminidase gene. Paramagnetic particles Dynabeads with covalently bound oligo (dT)25 were used as a tool for isolation of complementary H5N1 chains (H5N1 Zhejin, China and Aichi). For detection of H5N1 chains, oligonucleotide chains of lengths of 12 (+5 adenine) or 28 (+5 adenine) bp labeled with quantum dots (CdS, ZnS and/or PbS) were used. Individual probes hybridized to target molecules specifically with efficiency higher than 60%. The obtained signals identified mutations present in the sequence. Suggested experimental procedure allows obtaining further information from the redox signals of nucleic acids. Moreover, the used biosensor exhibits sequence specificity and low limits of detection of subnanogram quantities of target nucleic acids

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Návaznosti výsledku

  • Projekt

    Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

  • Návaznosti

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Ostatní

  • Rok uplatnění

    2013

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    VIRUSES-BASEL

  • ISSN

    1999-4915

  • e-ISSN

  • Svazek periodika

    5

  • Číslo periodika v rámci svazku

    7

  • Stát vydavatele periodika

    CH - Švýcarská konfederace

  • Počet stran výsledku

    21

  • Strana od-do

    1719-1739

  • Kód UT WoS článku

    000322172200009

  • EID výsledku v databázi Scopus