The importance of experimental milieu in regulation of macrophage activation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F13%3APU106106" target="_blank" >RIV/00216305:26620/13:PU106106 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The importance of experimental milieu in regulation of macrophage activation

Popis výsledku v původním jazyce

Background: Macrophages are important source of cytokines and other compounds, which recruit additional cells to sites of infection or tissue injury. Importantly, their functions might be directly controlled by the composition of milieu in their immediate vicinity. Therefore, the analysis of their function under different in vitro as well as simulated in vivo condition is of particular interest. Materials and methods: Mice peritoneal macrophages were stimulated with different activators (e.g. LPS, IFN-y, TNF-a and interleukins or their combination) under different experimental conditions (in vitro and simulated in vivo conditions). Consequently, the physiological functions of macrophages were assessed using different luminometric, spectrophotometric, molecular, and immunohistochemical methods. Results: Our data demonstrate that there exists crucial difference in activation of macrophages under classical in vitro and simulated in vivo conditions. These abnormalities are accompanied by significant changes in inflammatory response of macrophages as well as in activation of intracellular signaling pathways. Conclusions: The activation of mice peritoneal macrophages is dependent on the composition of milieu in their immediate vicinity and the classically used in vitro systems seems to be not suitable for correct analysis of their functions.

Název v anglickém jazyce

The importance of experimental milieu in regulation of macrophage activation

Popis výsledku anglicky

Background: Macrophages are important source of cytokines and other compounds, which recruit additional cells to sites of infection or tissue injury. Importantly, their functions might be directly controlled by the composition of milieu in their immediate vicinity. Therefore, the analysis of their function under different in vitro as well as simulated in vivo condition is of particular interest. Materials and methods: Mice peritoneal macrophages were stimulated with different activators (e.g. LPS, IFN-y, TNF-a and interleukins or their combination) under different experimental conditions (in vitro and simulated in vivo conditions). Consequently, the physiological functions of macrophages were assessed using different luminometric, spectrophotometric, molecular, and immunohistochemical methods. Results: Our data demonstrate that there exists crucial difference in activation of macrophages under classical in vitro and simulated in vivo conditions. These abnormalities are accompanied by significant changes in inflammatory response of macrophages as well as in activation of intracellular signaling pathways. Conclusions: The activation of mice peritoneal macrophages is dependent on the composition of milieu in their immediate vicinity and the classically used in vitro systems seems to be not suitable for correct analysis of their functions.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

EUROPEAN JOURNAL OF CLINICAL INVESTIGATION

ISSN

0014-2972

e-ISSN

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Svazek periodika

43

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

0-10

Kód UT WoS článku

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EID výsledku v databázi Scopus

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