The Role of Coherence in Image Formation in Holographic Microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F14%3APU109109" target="_blank" >RIV/00216305:26620/14:PU109109 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/B978-0-444-63379-8.00005-2" target="_blank" >http://dx.doi.org/10.1016/B978-0-444-63379-8.00005-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/B978-0-444-63379-8.00005-2" target="_blank" >10.1016/B978-0-444-63379-8.00005-2</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Role of Coherence in Image Formation in Holographic Microscopy

Popis výsledku v původním jazyce

Off-axis digital holographic microscopes (DHM) working with incoherent light have been designed and constructed. Their imaging properties can be changed by variation of the coherence of light. This spans from emulation of classic coherent-light DHM allowing for numerical focusing to incoherent-light DHM characterized by high-quality imaging, no coherence noise, halved limit of lateral resolution, and by coherence-gating effect making imaging in turbid media and optical sectioning possible. We describe theoretically the imaging process of a holographic microscope (HM) and how it is influenced by the coherence of illumination. The 3D coherent transfer function (CTF) reveals the dependence of a spatial frequency passband on the coherence properties of a source. Reduction of coherence leads to the passband broadening i.e. to the resolution enhancement. This effect is obvious also from the form of 3D point spread functions, which allows us to characterize imaging by 3D convolution. Imaging and numerical focusing of planar objects are described by 2D CTF derived from 3D CTF for various defocusing. Results for 2D objects are presented also in a simplified approximate form, which gives deeper insight into the fundaments of imaging. In this approximation, the image formation in a turbid medium by coherence gating is elucidated. In addition, it is shown that the mutual lateral shift of the object and reference beams amplifies higher spatial frequencies of a defocused object and allows an object in a turbid medium to be imaged by diffuse (non-ballistic) light. Important theoretical results are verified experimentally.

Název v anglickém jazyce

The Role of Coherence in Image Formation in Holographic Microscopy

Popis výsledku anglicky

Off-axis digital holographic microscopes (DHM) working with incoherent light have been designed and constructed. Their imaging properties can be changed by variation of the coherence of light. This spans from emulation of classic coherent-light DHM allowing for numerical focusing to incoherent-light DHM characterized by high-quality imaging, no coherence noise, halved limit of lateral resolution, and by coherence-gating effect making imaging in turbid media and optical sectioning possible. We describe theoretically the imaging process of a holographic microscope (HM) and how it is influenced by the coherence of illumination. The 3D coherent transfer function (CTF) reveals the dependence of a spatial frequency passband on the coherence properties of a source. Reduction of coherence leads to the passband broadening i.e. to the resolution enhancement. This effect is obvious also from the form of 3D point spread functions, which allows us to characterize imaging by 3D convolution. Imaging and numerical focusing of planar objects are described by 2D CTF derived from 3D CTF for various defocusing. Results for 2D objects are presented also in a simplified approximate form, which gives deeper insight into the fundaments of imaging. In this approximation, the image formation in a turbid medium by coherence gating is elucidated. In addition, it is shown that the mutual lateral shift of the object and reference beams amplifies higher spatial frequencies of a defocused object and allows an object in a turbid medium to be imaged by diffuse (non-ballistic) light. Important theoretical results are verified experimentally.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PROGRESS IN OPTICS

ISSN

0079-6638

e-ISSN

—

Svazek periodika

59

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

70

Strana od-do

267-336

Kód UT WoS článku

000348705400006

EID výsledku v databázi Scopus

2-s2.0-84899996688