Optimization of the Glutathione Detection by High Performance Liquid Chromatography with Electrochemical Detection in the Brain and Liver of Rats Fed with Taurin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F15%3APU113915" target="_blank" >RIV/00216305:26620/15:PU113915 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimization of the Glutathione Detection by High Performance Liquid Chromatography with Electrochemical Detection in the Brain and Liver of Rats Fed with Taurin

Popis výsledku v původním jazyce

Taurine exhibits many positive effects on organism, such as induction of important protective biochemical mechanisms and antioxidant effects. In this work, we studied the effect of taurine consumption on levels of taurine, reduced glutathione (GSH) and metallothionein in liver and brain tissues of Wistar rats. The aim of the experiment was the monitoring of changes in concentration of taurine in brain and liver tissues and also changes in concentration of GSH and metallothionein. After optimization, the limit of detection of GSH in brain and liver tissue was established as 40 nmol.g-1 of protein and 90 nmol.g-1 of protein, respectively. That is sufficient for determination of the concentration alterations of GSH in studied tissues. The consumption of taurine increased content of GSH determined in the brain and liver (increased by approximately 0.8 mmol.g-1 of protein in comparison with control). The increase of concentration of taurine occurred only in the brain, by cca 50 ug.g-1 of tissue, regardless the applied concentration of taurine. The applied concentration of taurine had no effect on concentration of metallothionein in any of the examined tissue.

Název v anglickém jazyce

Optimization of the Glutathione Detection by High Performance Liquid Chromatography with Electrochemical Detection in the Brain and Liver of Rats Fed with Taurin

Popis výsledku anglicky

Taurine exhibits many positive effects on organism, such as induction of important protective biochemical mechanisms and antioxidant effects. In this work, we studied the effect of taurine consumption on levels of taurine, reduced glutathione (GSH) and metallothionein in liver and brain tissues of Wistar rats. The aim of the experiment was the monitoring of changes in concentration of taurine in brain and liver tissues and also changes in concentration of GSH and metallothionein. After optimization, the limit of detection of GSH in brain and liver tissue was established as 40 nmol.g-1 of protein and 90 nmol.g-1 of protein, respectively. That is sufficient for determination of the concentration alterations of GSH in studied tissues. The consumption of taurine increased content of GSH determined in the brain and liver (increased by approximately 0.8 mmol.g-1 of protein in comparison with control). The increase of concentration of taurine occurred only in the brain, by cca 50 ug.g-1 of tissue, regardless the applied concentration of taurine. The applied concentration of taurine had no effect on concentration of metallothionein in any of the examined tissue.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

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OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/ED2.1.00%2F03.0072" target="_blank" >ED2.1.00/03.0072: Centrum senzorických, informačních a komunikačních systémů (SIX)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

ISSN

1452-3981

e-ISSN

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Svazek periodika

10

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

RS - Srbská republika

Počet stran výsledku

12

Strana od-do

1716-1727

Kód UT WoS článku

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EID výsledku v databázi Scopus

2-s2.0-84921632639