Optimization of the Glutathione Detection by High Performance Liquid Chromatography with Electrochemical Detection in the Brain and Liver of Rats Fed with Taurine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906310" target="_blank" >RIV/62156489:43210/15:43906310 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43410/15:43906310 RIV/00216305:26220/14:PU116443

Výsledek na webu

<a href="http://www.electrochemsci.org/papers/vol10/100201716.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol10/100201716.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimization of the Glutathione Detection by High Performance Liquid Chromatography with Electrochemical Detection in the Brain and Liver of Rats Fed with Taurine

Popis výsledku v původním jazyce

Taurine exhibits many positive effects on organism, such as induction of important protective biochemical mechanisms and antioxidant effects. In this work, we studied the effect of taurine consumption on levels of taurine, reduced glutathione (GSH) and metallothionein in liver and brain tissues of Wistar rats. The aim of the experiment was the monitoring of changes in concentration of taurine in brain and liver tissues and also changes in concentration of GSH and metallothionein. After optimization, thelimit of detection of GSH in brain and liver tissue was established as 40 nmol.g(-1) of protein and 90 nmol.g(-1) of protein, respectively. That is sufficient for determination of the concentration alterations of GSH in studied tissues. The consumptionof taurine increased content of GSH determined in the brain and liver (increased by approximately 0.8 mmol.g(-1) of protein in comparison with control). The increase of concentration of taurine occurred only in the brain, by cca 50 mu g.g

Název v anglickém jazyce

Optimization of the Glutathione Detection by High Performance Liquid Chromatography with Electrochemical Detection in the Brain and Liver of Rats Fed with Taurine

Popis výsledku anglicky

Taurine exhibits many positive effects on organism, such as induction of important protective biochemical mechanisms and antioxidant effects. In this work, we studied the effect of taurine consumption on levels of taurine, reduced glutathione (GSH) and metallothionein in liver and brain tissues of Wistar rats. The aim of the experiment was the monitoring of changes in concentration of taurine in brain and liver tissues and also changes in concentration of GSH and metallothionein. After optimization, thelimit of detection of GSH in brain and liver tissue was established as 40 nmol.g(-1) of protein and 90 nmol.g(-1) of protein, respectively. That is sufficient for determination of the concentration alterations of GSH in studied tissues. The consumptionof taurine increased content of GSH determined in the brain and liver (increased by approximately 0.8 mmol.g(-1) of protein in comparison with control). The increase of concentration of taurine occurred only in the brain, by cca 50 mu g.g

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GH - Výživa hospodářských zvířat

OECD FORD obor

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Projekt

<a href="/cs/project/ED2.1.00%2F03.0072" target="_blank" >ED2.1.00/03.0072: Centrum senzorických, informačních a komunikačních systémů (SIX)</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Svazek periodika

10

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CS - Srbsko a Černá Hora

Počet stran výsledku

12

Strana od-do

1716-1727

Kód UT WoS článku

000348365800054

EID výsledku v databázi Scopus

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