Industrial Tomography System for Answering Biological Issues: Development of the Mouse Embryo Face.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F16%3APU118925" target="_blank" >RIV/00216305:26620/16:PU118925 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.ndt.net/search/docs.php3?id=18758" target="_blank" >http://www.ndt.net/search/docs.php3?id=18758</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Industrial Tomography System for Answering Biological Issues: Development of the Mouse Embryo Face.

Popis výsledku v původním jazyce

Understanding of some biological processes like a face development requires among others the high-resolution 3D imaging of increasingly complex cartilage in verterate embryos. During the face development, cartilages are produced in a variety of shapes and sizes making a convenient model system. X-ray computed microtomography (microCT) imaging is limited by the low inherent contrast of non-mineralized tissues. Although X-ray contrast enhancement agents are used routinely in clinical radiography, only a few techniques have appeared for imaging soft tissues in preserved animal specimens. Here we present a feasibility study of three different tomographic systems for high resolution and high contrast imaging of embryonic tissues. The industrial system equipped with flat-panel is compared with microCT system using synchrotron radiation (SYRMEP beamline of Elettra, Italy) and laboratory-based micro/nano CT system (Rigaku, nano3DX) based on Mo target and CCD camera. The mouse embryos were stained by phosphotungstic acid which produced overall contrast and differential tissue contrast. Furthermore, we demonstrate the utilisation of the standard industrial tools (wall thickness analysis and 3D printing) which can help to understand the differences or similarities among of different mouse embryonic development stages.

Název v anglickém jazyce

Industrial Tomography System for Answering Biological Issues: Development of the Mouse Embryo Face.

Popis výsledku anglicky

Understanding of some biological processes like a face development requires among others the high-resolution 3D imaging of increasingly complex cartilage in verterate embryos. During the face development, cartilages are produced in a variety of shapes and sizes making a convenient model system. X-ray computed microtomography (microCT) imaging is limited by the low inherent contrast of non-mineralized tissues. Although X-ray contrast enhancement agents are used routinely in clinical radiography, only a few techniques have appeared for imaging soft tissues in preserved animal specimens. Here we present a feasibility study of three different tomographic systems for high resolution and high contrast imaging of embryonic tissues. The industrial system equipped with flat-panel is compared with microCT system using synchrotron radiation (SYRMEP beamline of Elettra, Italy) and laboratory-based micro/nano CT system (Rigaku, nano3DX) based on Mo target and CCD camera. The mouse embryos were stained by phosphotungstic acid which produced overall contrast and differential tissue contrast. Furthermore, we demonstrate the utilisation of the standard industrial tools (wall thickness analysis and 3D printing) which can help to understand the differences or similarities among of different mouse embryonic development stages.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JR - Ostatní strojírenství

OECD FORD obor

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Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

The e-Journal of Nondestructive Testing

ISSN

1435-4934

e-ISSN

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Svazek periodika

Neuveden

Číslo periodika v rámci svazku

2016

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

1-9

Kód UT WoS článku

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EID výsledku v databázi Scopus

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