Coherence-controlled holographic microscopy for live-cell quantitative phase imaging in turbid media

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F16%3APU125847" target="_blank" >RIV/00216305:26620/16:PU125847 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.spiedigitallibrary.org/conference-proceedings-of-spie/9718/1/Coherence-controlled-holographic-microscopy-for-live-cell-quantitative-phase-imaging/10.1117/12.2209322.short" target="_blank" >https://www.spiedigitallibrary.org/conference-proceedings-of-spie/9718/1/Coherence-controlled-holographic-microscopy-for-live-cell-quantitative-phase-imaging/10.1117/12.2209322.short</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1117/12.2209322" target="_blank" >10.1117/12.2209322</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Coherence-controlled holographic microscopy for live-cell quantitative phase imaging in turbid media

Popis výsledku v původním jazyce

In this work we present the coherence controlled holographic microscopy (CCHM)(1) and its ability to image the living cells in turbid media(2). The CCHM method and its advantages are introduced. A 'coherence gate effect'(3), that enables imaging in turbid media, occurs owing to the low coherence illumination in our setup. The coherence gate effect is briefly theoretically explained and comparison of images with different illumination sources is shown. After that, the possibility of imaging in turbid media is applied to investigation of cell reactions to cytopathic turbid emulsions. In our experiments we used human cancer cells treated by biologically active phospholipids (BAPs). Cellular events leading to cell death, that would otherwise remain hidden in turbid media, are clearly observable and according to them cell fate can be deduced.

Název v anglickém jazyce

Coherence-controlled holographic microscopy for live-cell quantitative phase imaging in turbid media

Popis výsledku anglicky

In this work we present the coherence controlled holographic microscopy (CCHM)(1) and its ability to image the living cells in turbid media(2). The CCHM method and its advantages are introduced. A 'coherence gate effect'(3), that enables imaging in turbid media, occurs owing to the low coherence illumination in our setup. The coherence gate effect is briefly theoretically explained and comparison of images with different illumination sources is shown. After that, the possibility of imaging in turbid media is applied to investigation of cell reactions to cytopathic turbid emulsions. In our experiments we used human cancer cells treated by biologically active phospholipids (BAPs). Cellular events leading to cell death, that would otherwise remain hidden in turbid media, are clearly observable and according to them cell fate can be deduced.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

QUANTITATIVE PHASE IMAGING II

ISBN

978-1-62841-952-8

ISSN

0277-786X

e-ISSN

—

Počet stran výsledku

5

Strana od-do

„97181Y-1“-„97181Y-5“

Název nakladatele

Neuveden

Místo vydání

Neuveden

Místo konání akce

San Francisco

Datum konání akce

13. 2. 2016

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000380605300044