A Perspective Combination Quantum Dots and Silica Nanoparticies: for Bioconjugation with Antibodies used in Immunosensors for Detection of Human Alpha-Fetoprotein

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A Perspective Combination Quantum Dots and Silica Nanoparticies: for Bioconjugation with Antibodies used in Immunosensors for Detection of Human Alpha-Fetoprotein

Popis výsledku v původním jazyce

The combination of quantum dots (QDs) with silica nanoparticles (SiNPs) as promising signal-generating label of antibodies used in electrochemical immunosensor is described. QDs-modified silica nanoparticles were conjugated with specific antibodies targeted to alpha-fetoprotein (AFP) to a creation of anti-AFP-SiNPs-QDs IgG conjugate for subsequent exploitation in quantum dot-linked immunosorbent assay (QLISA). Square wave anodic stripping voltammetry (SWASV) on mercury or bismuth film modified carbon screen-printed three-electrode sensors was used as a detection method. Insertion of nanoparticles to this system led to the achievement of higher current responses compare to results obtained with QDs only. Therefore, the sensitivity of complete immunoassay could be significantly improved.

Název v anglickém jazyce

A Perspective Combination Quantum Dots and Silica Nanoparticies: for Bioconjugation with Antibodies used in Immunosensors for Detection of Human Alpha-Fetoprotein

Popis výsledku anglicky

The combination of quantum dots (QDs) with silica nanoparticles (SiNPs) as promising signal-generating label of antibodies used in electrochemical immunosensor is described. QDs-modified silica nanoparticles were conjugated with specific antibodies targeted to alpha-fetoprotein (AFP) to a creation of anti-AFP-SiNPs-QDs IgG conjugate for subsequent exploitation in quantum dot-linked immunosorbent assay (QLISA). Square wave anodic stripping voltammetry (SWASV) on mercury or bismuth film modified carbon screen-printed three-electrode sensors was used as a detection method. Insertion of nanoparticles to this system led to the achievement of higher current responses compare to results obtained with QDs only. Therefore, the sensitivity of complete immunoassay could be significantly improved.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XXXVII MODERNI ELEKTROCHEMICKE METODY

ISBN

978-80-905221-5-2

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

108-112

Název nakladatele

Neuveden

Místo vydání

Neuveden

Místo konání akce

Jetřichovice

Datum konání akce

15. 5. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000432424300024