Differences in siRNA encapsulation between HsaHFt-RK ferritin and EcaLHFt

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU134911" target="_blank" >RIV/00216305:26620/19:PU134911 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/19:43917123

Výsledek na webu

<a href="https://mendelnet.cz/artkey/mnt-201901-0110_Differences-in-siRNA-encapsulation-between-HsaHFt-RK-ferritin-and-EcaLHFt.php?back=/magno/mnt/2019/mn1.php?secid=4" target="_blank" >https://mendelnet.cz/artkey/mnt-201901-0110_Differences-in-siRNA-encapsulation-between-HsaHFt-RK-ferritin-and-EcaLHFt.php?back=/magno/mnt/2019/mn1.php?secid=4</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Differences in siRNA encapsulation between HsaHFt-RK ferritin and EcaLHFt

Popis výsledku v původním jazyce

Nanomedicine in cancer treatment has a great potential. With usage of proper nanocarrier we would be able to eliminate negative side effects and excessively high toxicity of chemotherapeutical drugs to normal cells. Ferritins in general are proteins which could do the trick. Their ability to change structure in low pH could be used for drug delivery. Ferritins are also ubiquitous so there is also very small chance of immune reaction in body. First and the most used and described ferritin is EcaLHFt (ferritin from horse spleen). Its ability to encapsulate various molecules is already known and used in research laboratories. Only molecules with negative charge are troublesome to encapsulate into EcaLHFt. For this reason, we created recombinant human ferritin – HsaHFt-RK, which is designed to encapsulate predominantly negatively charged molecules. We were able to confirm this hypothesis by comparing the encapsulation efficiency of FAM-siRNA inside EcaLHFt and HsaHFt-RK.

Název v anglickém jazyce

Differences in siRNA encapsulation between HsaHFt-RK ferritin and EcaLHFt

Popis výsledku anglicky

Nanomedicine in cancer treatment has a great potential. With usage of proper nanocarrier we would be able to eliminate negative side effects and excessively high toxicity of chemotherapeutical drugs to normal cells. Ferritins in general are proteins which could do the trick. Their ability to change structure in low pH could be used for drug delivery. Ferritins are also ubiquitous so there is also very small chance of immune reaction in body. First and the most used and described ferritin is EcaLHFt (ferritin from horse spleen). Its ability to encapsulate various molecules is already known and used in research laboratories. Only molecules with negative charge are troublesome to encapsulate into EcaLHFt. For this reason, we created recombinant human ferritin – HsaHFt-RK, which is designed to encapsulate predominantly negatively charged molecules. We were able to confirm this hypothesis by comparing the encapsulation efficiency of FAM-siRNA inside EcaLHFt and HsaHFt-RK.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/GA17-12816S" target="_blank" >GA17-12816S: Konstrukce modifikovaných apoferitinových nanočástic s protinádorovými léčivy a studium mechanismů potencujících jejich efektivitu v terapii</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2019 Proceedings of 26th International PhD Students Conference

ISBN

978-80-7509-688-3

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

551-556

Název nakladatele

Mendel University in Brno

Místo vydání

Neuveden

Místo konání akce

Brno

Datum konání akce

6. 11. 2019

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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