Possitively charged ferritin nanocages can deliver sirna into cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F20%3APU139200" target="_blank" >RIV/00216305:26620/20:PU139200 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.11159/icnfa20.122" target="_blank" >http://dx.doi.org/10.11159/icnfa20.122</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Possitively charged ferritin nanocages can deliver sirna into cells

Popis výsledku v původním jazyce

Nanomedicine is based on using nanotransporters for transfer of drugs and other active compounds inside cancer tissue without damaging the normal cells and organs [1]. For this purpose, various types of molecules can be used. Using proteins as nanocarriers is tested for their biocompatibility, safety and non-toxicity [2]. Interested protein based nanotransporter is ferritin. It is ubiquitous, with convenient size, robust, stable at various conditions and it is easy to modify its surface to achieve active targeting [3]. However, its best quality is the ability to open the protein cage in pH below 3 and folding it back together in pH above 7 [4]. This property is used for active encapsulation of various molecules. By mixing ferritin with the compounds of interest, lowering pH with HCl and then returning pH back to neutral by addition of NaOH, we can encapsulated cargo inside ferritin cage [5]. Recombinant human ferritin (HsaFtH-RK) was designed and cloned under supervision of Dr. Pekarik. The production

Název v anglickém jazyce

Possitively charged ferritin nanocages can deliver sirna into cells

Popis výsledku anglicky

Nanomedicine is based on using nanotransporters for transfer of drugs and other active compounds inside cancer tissue without damaging the normal cells and organs [1]. For this purpose, various types of molecules can be used. Using proteins as nanocarriers is tested for their biocompatibility, safety and non-toxicity [2]. Interested protein based nanotransporter is ferritin. It is ubiquitous, with convenient size, robust, stable at various conditions and it is easy to modify its surface to achieve active targeting [3]. However, its best quality is the ability to open the protein cage in pH below 3 and folding it back together in pH above 7 [4]. This property is used for active encapsulation of various molecules. By mixing ferritin with the compounds of interest, lowering pH with HCl and then returning pH back to neutral by addition of NaOH, we can encapsulated cargo inside ferritin cage [5]. Recombinant human ferritin (HsaFtH-RK) was designed and cloned under supervision of Dr. Pekarik. The production

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů