Optimization of multiplex RT-PCR for selected isoforms of metallothionein genes and influence of cisplatin on prostatic cell lines

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU134919" target="_blank" >RIV/00216305:26620/19:PU134919 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/19:43917134

Výsledek na webu

<a href="https://mendelnet.cz/artkey/mnt-201901-0117_Optimization-of-multiplex-RT-PCR-for-selected-isoforms-of-metallothionein-genes-and-influence-of-cisplatin-on-p.php?back=/magno/mnt/2019/mn1.php?secid=4" target="_blank" >https://mendelnet.cz/artkey/mnt-201901-0117_Optimization-of-multiplex-RT-PCR-for-selected-isoforms-of-metallothionein-genes-and-influence-of-cisplatin-on-p.php?back=/magno/mnt/2019/mn1.php?secid=4</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Optimization of multiplex RT-PCR for selected isoforms of metallothionein genes and influence of cisplatin on prostatic cell lines

Popis výsledku v původním jazyce

This study deals with the issue of metallothioneins as potential tumor markers using the multiplex reverse transcription polymerase chain reaction (RT-PCR) method, for which specific primers were designed, with the subsequent sequencing of their products. The following step involved a study of expression using the PCR method for selected MT1A, MT2A and MT3 genes after treating cell lines with cisplatin. Experiments were carried out on PNT1A, LNCaP and DU145 prostatic cell lines. Despite a very high similarity of the individual isoforms of metallothioneins, a triplex of three genes for two cell lines, PNT1A and DU145, was created. Upon a statistical evaluation of data obtained via the method of polymerase chain reaction in real time, it was found that, after the application of cisplatin, there was an increase in metallothionein expression.

Název v anglickém jazyce

Optimization of multiplex RT-PCR for selected isoforms of metallothionein genes and influence of cisplatin on prostatic cell lines

Popis výsledku anglicky

This study deals with the issue of metallothioneins as potential tumor markers using the multiplex reverse transcription polymerase chain reaction (RT-PCR) method, for which specific primers were designed, with the subsequent sequencing of their products. The following step involved a study of expression using the PCR method for selected MT1A, MT2A and MT3 genes after treating cell lines with cisplatin. Experiments were carried out on PNT1A, LNCaP and DU145 prostatic cell lines. Despite a very high similarity of the individual isoforms of metallothioneins, a triplex of three genes for two cell lines, PNT1A and DU145, was created. Upon a statistical evaluation of data obtained via the method of polymerase chain reaction in real time, it was found that, after the application of cisplatin, there was an increase in metallothionein expression.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

30204 - Oncology

Projekt

<a href="/cs/project/GC19-13766J" target="_blank" >GC19-13766J: Zinek-dependentní signalizace a exprese sub/isoforem metalothioneinu v karcinomu prsu: implikace pro prognostické a terapeutické účely</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2019 Proceedings of 26th International PhD Students Conference

ISBN

978-80-7509-688-3

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

606-611

Název nakladatele

Mendel University in Brno

Místo vydání

Neuveden

Místo konání akce

Brno

Datum konání akce

6. 11. 2019

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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