Sensitive biosensor for detection oncogenic miRNA-21
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU134936" target="_blank" >RIV/00216305:26620/19:PU134936 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62156489:43210/19:43917158
Výsledek na webu
<a href="https://mendelnet.cz/artkey/mnt-201901-0130_Sensitive-biosensor-for-detection-oncogenic-miRNA-21.php?back=/magno/mnt/2019/mn1.php?secid=4" target="_blank" >https://mendelnet.cz/artkey/mnt-201901-0130_Sensitive-biosensor-for-detection-oncogenic-miRNA-21.php?back=/magno/mnt/2019/mn1.php?secid=4</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Sensitive biosensor for detection oncogenic miRNA-21
Popis výsledku v původním jazyce
The aim of this study was to prepare a sensitive electrochemical biosensor for detection of oncogenic miRNA-21 as a potential biomarker for early detection of cancer. MicroRNAs (miRNAs) are small noncoding RNA, which play an important role in the regulation of gene expression. miRNAs are present in extracellular fluids like urine, serum, etc. in extremely low concentration. It is important for the biosensor to detect extremely low concentrations of specific miRNA as potential biomarkers. The aim of this experiment was designing, preparing and optimizing a sensitive and selective biosensor for the detection of miRNA-21. A biosensor was prepared to detect the lowest concentration of miRNA-21 in the sample. The linear concentration range for the calibration curve was 1 fM to 10 nM. LOD was 1 fM and obtained from the regression equation was 3.2 zM and LOQ was 10.8 zM. Subsequently, prepared biosensor was measured in artificial urine (AU) samples to verify the functioning of the sensor in induced real conditions. The results showed a minimal effect of the matrix for the determination of the target miRNA.
Název v anglickém jazyce
Sensitive biosensor for detection oncogenic miRNA-21
Popis výsledku anglicky
The aim of this study was to prepare a sensitive electrochemical biosensor for detection of oncogenic miRNA-21 as a potential biomarker for early detection of cancer. MicroRNAs (miRNAs) are small noncoding RNA, which play an important role in the regulation of gene expression. miRNAs are present in extracellular fluids like urine, serum, etc. in extremely low concentration. It is important for the biosensor to detect extremely low concentrations of specific miRNA as potential biomarkers. The aim of this experiment was designing, preparing and optimizing a sensitive and selective biosensor for the detection of miRNA-21. A biosensor was prepared to detect the lowest concentration of miRNA-21 in the sample. The linear concentration range for the calibration curve was 1 fM to 10 nM. LOD was 1 fM and obtained from the regression equation was 3.2 zM and LOQ was 10.8 zM. Subsequently, prepared biosensor was measured in artificial urine (AU) samples to verify the functioning of the sensor in induced real conditions. The results showed a minimal effect of the matrix for the determination of the target miRNA.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
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OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2019
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
MendelNet 2019 Proceedings of 26th International PhD Students Conference
ISBN
978-80-7509-688-3
ISSN
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e-ISSN
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Počet stran výsledku
6
Strana od-do
676-681
Název nakladatele
Mendel University in Brno
Místo vydání
Neuveden
Místo konání akce
Brno
Datum konání akce
6. 11. 2019
Typ akce podle státní příslušnosti
CST - Celostátní akce
Kód UT WoS článku
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