Highly selective and sensitive electrochemical biosensor based on electrochemically reduced graphene oxide for detection of miRNA as a cancer biomarker

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43917970" target="_blank" >RIV/62156489:43210/20:43917970 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/20:PU136652

Výsledek na webu

<a href="https://doi.org/10.37904/nanocon.2019.8615" target="_blank" >https://doi.org/10.37904/nanocon.2019.8615</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2019.8615" target="_blank" >10.37904/nanocon.2019.8615</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Highly selective and sensitive electrochemical biosensor based on electrochemically reduced graphene oxide for detection of miRNA as a cancer biomarker

Popis výsledku v původním jazyce

Nowadays, cancer is one of the most outrageous diseases affecting millions of people throughout the world. In this study, the sensitive biosensor for miRNA-21 detection was developed. Through a facile sulfonamide coupling reaction a miRNA-21 specific DNA hybridization probe was immobilized onto a glassy carbon electrode (GCE) modified with electrochemical reduced graphene oxide (erGO). miRNA is a small non-coding RNA molecule which plays an important role in gene expression. The level of miRNA in extracellular fluids varying in the presence of cancer and it can serve as creates a potential biomarker for early detection of cancer. A higher levelcancer. An elevated level of miRNA-21 represents a common feature of pathological cell growth or cell stress and is connected with almost all types of cancer and with cardiovascular diseases, as well.The aim of this study was improve the electrochemical properties by using erGO as a modification for better sensitivity of the developed biosensor. After the modification with erGO, 1-amino-2-naphthol-4-sulfonic acid (AN-SO3-) was electrodeposited on the GCE surface.Then the GCE was subsequently modified by electrochemical deposition 1-amino-2-naphthol-4-sulfonic acid (AN-SO3-) on the GCE surface. Finally, the amino terminated capture probe was immobilized on the GCE via the sulfonamide coupling reaction. The developed biosensor for the detection of specific miRNA sequence was optimized and modified for using in further experiments.

Název v anglickém jazyce

Highly selective and sensitive electrochemical biosensor based on electrochemically reduced graphene oxide for detection of miRNA as a cancer biomarker

Popis výsledku anglicky

Nowadays, cancer is one of the most outrageous diseases affecting millions of people throughout the world. In this study, the sensitive biosensor for miRNA-21 detection was developed. Through a facile sulfonamide coupling reaction a miRNA-21 specific DNA hybridization probe was immobilized onto a glassy carbon electrode (GCE) modified with electrochemical reduced graphene oxide (erGO). miRNA is a small non-coding RNA molecule which plays an important role in gene expression. The level of miRNA in extracellular fluids varying in the presence of cancer and it can serve as creates a potential biomarker for early detection of cancer. A higher levelcancer. An elevated level of miRNA-21 represents a common feature of pathological cell growth or cell stress and is connected with almost all types of cancer and with cardiovascular diseases, as well.The aim of this study was improve the electrochemical properties by using erGO as a modification for better sensitivity of the developed biosensor. After the modification with erGO, 1-amino-2-naphthol-4-sulfonic acid (AN-SO3-) was electrodeposited on the GCE surface.Then the GCE was subsequently modified by electrochemical deposition 1-amino-2-naphthol-4-sulfonic acid (AN-SO3-) on the GCE surface. Finally, the amino terminated capture probe was immobilized on the GCE via the sulfonamide coupling reaction. The developed biosensor for the detection of specific miRNA sequence was optimized and modified for using in further experiments.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON 2019: Conference Proceedings

ISBN

978-80-87294-95-6

ISSN

2694-930X

e-ISSN

—

Počet stran výsledku

6

Strana od-do

385-390

Název nakladatele

Tanger Ltd.

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

16. 10. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000664115400064