Development of electrochemical biosensor for direct histamine detection by using selective peptides as biorecognition element

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919619" target="_blank" >RIV/62156489:43210/20:43919619 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Development of electrochemical biosensor for direct histamine detection by using selective peptides as biorecognition element

Popis výsledku v původním jazyce

The level of histamine (HIS) in different food sources is critical as an indicator of food quality and safety. In this context, the proper monitoring of HIS in food is necessary. Electrochemical biosensors, with the possibility of fast, sensitive, and portable technology, represent a new promising approach for direct detection of HIS. Moreover, peptides as biorecognition elements are promising due to their unique properties such as good stability, specific and selective binding to various biomolecules. In this study, the fabrication and optimization of an electrochemical biosensor for direct detection of HIS based-on peptides as recognition elements, is presented. The specific HIS binding peptides (HBP) were immobilized via a sulphonamide bond on 1-amino-2-naphthol-4-sulfonic acid (ANSA), which was electrodeposited on a glassy carbon electrode (GCE). Furthermore, the optimization of the surface coverage with the peptide probe as well as the effect of the pH conditions for HIS binding was investigated. The measured concentrations were from 10 nM (10MINUS SIGN 8 M) to 1 mM (10MINUS SIGN 3 M) and LOD was calculated as 6.53 nM and LOQ as 21.75 mM.

Název v anglickém jazyce

Development of electrochemical biosensor for direct histamine detection by using selective peptides as biorecognition element

Popis výsledku anglicky

The level of histamine (HIS) in different food sources is critical as an indicator of food quality and safety. In this context, the proper monitoring of HIS in food is necessary. Electrochemical biosensors, with the possibility of fast, sensitive, and portable technology, represent a new promising approach for direct detection of HIS. Moreover, peptides as biorecognition elements are promising due to their unique properties such as good stability, specific and selective binding to various biomolecules. In this study, the fabrication and optimization of an electrochemical biosensor for direct detection of HIS based-on peptides as recognition elements, is presented. The specific HIS binding peptides (HBP) were immobilized via a sulphonamide bond on 1-amino-2-naphthol-4-sulfonic acid (ANSA), which was electrodeposited on a glassy carbon electrode (GCE). Furthermore, the optimization of the surface coverage with the peptide probe as well as the effect of the pH conditions for HIS binding was investigated. The measured concentrations were from 10 nM (10MINUS SIGN 8 M) to 1 mM (10MINUS SIGN 3 M) and LOD was calculated as 6.53 nM and LOQ as 21.75 mM.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2020: Proceedings of International PhD Students Conference

ISBN

978-80-7509-765-1

ISSN

—

e-ISSN

—

Počet stran výsledku

6

Strana od-do

578-583

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2020

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

—