Amine oxidase based biosensor for histamine detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F00%3A00001152" target="_blank" >RIV/61989592:15310/00:00001152 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Amine oxidase based biosensor for histamine detection

Popis výsledku v původním jazyce

This work reports on the development and optimization of amperometric biosensors based on the enyzme amine oxidase (AO) for the detection of histamine, a well-known biomarker for food freshness. Biosensor characteristics were evaluated in a flow injection (FI) analysis line, operated at +200 mV (vs. Ag/AgCl/0.1 M KCl). Two different biosensor designs were considered, one based on adsorbed AO on graphite electrodes, the detection being based on a direct electron transfer (DET) mechanism, whereas the second one based on a Os-bipyridine modified redox polymer using a mediated electron transfer (MET) pathway. Both electrode designs were able to detect histamine in mM range, however, the [osmium(4,4´-dimethylbipyridine)2Cl]+/2+ complexed with poly(1-vinylimidazole) (PVI13-dmeOs) based electrodes showed superior characteristics with regard to stability, selectivity and linear range. These electrodes were characterized by a detection limit of 2.2 mM (calculated as three times the signal-to-no

Název v anglickém jazyce

Amine oxidase based biosensor for histamine detection

Popis výsledku anglicky

This work reports on the development and optimization of amperometric biosensors based on the enyzme amine oxidase (AO) for the detection of histamine, a well-known biomarker for food freshness. Biosensor characteristics were evaluated in a flow injection (FI) analysis line, operated at +200 mV (vs. Ag/AgCl/0.1 M KCl). Two different biosensor designs were considered, one based on adsorbed AO on graphite electrodes, the detection being based on a direct electron transfer (DET) mechanism, whereas the second one based on a Os-bipyridine modified redox polymer using a mediated electron transfer (MET) pathway. Both electrode designs were able to detect histamine in mM range, however, the [osmium(4,4´-dimethylbipyridine)2Cl]+/2+ complexed with poly(1-vinylimidazole) (PVI13-dmeOs) based electrodes showed superior characteristics with regard to stability, selectivity and linear range. These electrodes were characterized by a detection limit of 2.2 mM (calculated as three times the signal-to-no

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F97%2F0097" target="_blank" >GA203/97/0097: Molekulární, strukturní a fyziologické vlastnosti rostlinných aminoxidas a cytokininoxidas</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

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Svazek periodika

12

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

XX - osoby bez státní příslušnosti

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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