Biosensors for detection of histamine and other amines based on amine oxidase modified graphite electrode

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F00%3A00001059" target="_blank" >RIV/61989592:15310/00:00001059 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Biosensors for detection of histamine and other amines based on amine oxidase modified graphite electrode

Popis výsledku v původním jazyce

Electrochemical biosensors based on graphite electrode modified either with copper amine oxidase (EC 1.4.3.6) from grass pea (Lathyrus sativus) or with the amine oxidase coupled with horseradish peroxidase (EC 1.11.1.7) were constructed and used for thedetermination of biogenic amines (histamine, putrescine, cadaverine, tyramine, cystamine, agmatine, spermidine, ethylenediamine, etc.) commonly present in food products. The amine oxidase was effective working both in mono- and bienzyme electrode designsbased either on a direct or mediated electron transfer approach. However the two biosensor designs displayed different selectivity patterns, allowing thus the simultaneous assay of histamine and putrescine. The reaction mechanism of the monoenzymatic unmediated biosensor shows unique features of direct electron transfer achieved for the first time for the copper amine oxidase. The biosensors characteristics obtained by simple adsorption of the enzymes on the graphite surface were compar

Název v anglickém jazyce

Biosensors for detection of histamine and other amines based on amine oxidase modified graphite electrode

Popis výsledku anglicky

Electrochemical biosensors based on graphite electrode modified either with copper amine oxidase (EC 1.4.3.6) from grass pea (Lathyrus sativus) or with the amine oxidase coupled with horseradish peroxidase (EC 1.11.1.7) were constructed and used for thedetermination of biogenic amines (histamine, putrescine, cadaverine, tyramine, cystamine, agmatine, spermidine, ethylenediamine, etc.) commonly present in food products. The amine oxidase was effective working both in mono- and bienzyme electrode designsbased either on a direct or mediated electron transfer approach. However the two biosensor designs displayed different selectivity patterns, allowing thus the simultaneous assay of histamine and putrescine. The reaction mechanism of the monoenzymatic unmediated biosensor shows unique features of direct electron transfer achieved for the first time for the copper amine oxidase. The biosensors characteristics obtained by simple adsorption of the enzymes on the graphite surface were compar

Druh

C - Kapitola v odborné knize

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

29th Annual Meeting of the European Histamine Research Society

ISBN

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Počet stran výsledku

97

Strana od-do

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Počet stran knihy

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Název nakladatele

European Histamine Research Society

Místo vydání

Rome, Italy

Kód UT WoS kapitoly

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