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Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F21%3APU140946" target="_blank" >RIV/00216305:26620/21:PU140946 - isvavai.cz</a>

  • Nalezeny alternativní kódy

    RIV/62156489:43210/21:43919859

  • Výsledek na webu

    <a href="https://doi.org/10.1016/j.snb.2021.129999" target="_blank" >https://doi.org/10.1016/j.snb.2021.129999</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.snb.2021.129999" target="_blank" >10.1016/j.snb.2021.129999</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers

  • Popis výsledku v původním jazyce

    Microfluidic paper-based analytical devices (mu PADs) offer the advantages of simplicity, extremely low costs, robustness, and miniaturisation, synergistically supporting portability and point-of-care (POC) analysis. When mu PADs are combined with distance-based detection in D mu PADs, they uniquely enable a quantitative analytical platform that is truly instrumentation-free (naked-eye readout), or at least, does not require any specialised scientific instrumentation (only mobile phone camera). However, a significant drawback of D mu PADs is their limited selectivity. In this work, we present for the first time molecularly imprinted polymer (MIP) as a selectivity-enhancing element in MIP-modified D mu PADs (MIP-D mu PADs). Herein, a layer of polydopamine MIP was coated onto the paper substrate of a D mu PAD, in a simple process using dopamine as the monomer deposited onto the paper matrix in the migration-detection zone of the D mu PAD, and polymerised in a rapid low cost procedure in the presence of oxygen under alkaline conditions. The polydopamine MIP-D mu PAD was then systematically investigated for the selective determination of chymotrypsinogen (chymo) as a model protein biomarker in urine, within the linear concentration range 2.4-29.2 mu M (R2 = 0,9903) with corresponding relative standard deviations ranging from 2% to 11 % and LOD =3.5 mu M and LOQ =11.8 mu M. The here presented analytical concept based on MIP-D mu PADs has a potential in POC diagnostics, because of the combination of low cost automated fabrication, the rapid quantitative near to instrumentation-free analysis, and selectivity through the use of MIPs as a synthetic, more stable, cheaper and easily prepared alternative to bio-macromolecules.

  • Název v anglickém jazyce

    Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers

  • Popis výsledku anglicky

    Microfluidic paper-based analytical devices (mu PADs) offer the advantages of simplicity, extremely low costs, robustness, and miniaturisation, synergistically supporting portability and point-of-care (POC) analysis. When mu PADs are combined with distance-based detection in D mu PADs, they uniquely enable a quantitative analytical platform that is truly instrumentation-free (naked-eye readout), or at least, does not require any specialised scientific instrumentation (only mobile phone camera). However, a significant drawback of D mu PADs is their limited selectivity. In this work, we present for the first time molecularly imprinted polymer (MIP) as a selectivity-enhancing element in MIP-modified D mu PADs (MIP-D mu PADs). Herein, a layer of polydopamine MIP was coated onto the paper substrate of a D mu PAD, in a simple process using dopamine as the monomer deposited onto the paper matrix in the migration-detection zone of the D mu PAD, and polymerised in a rapid low cost procedure in the presence of oxygen under alkaline conditions. The polydopamine MIP-D mu PAD was then systematically investigated for the selective determination of chymotrypsinogen (chymo) as a model protein biomarker in urine, within the linear concentration range 2.4-29.2 mu M (R2 = 0,9903) with corresponding relative standard deviations ranging from 2% to 11 % and LOD =3.5 mu M and LOQ =11.8 mu M. The here presented analytical concept based on MIP-D mu PADs has a potential in POC diagnostics, because of the combination of low cost automated fabrication, the rapid quantitative near to instrumentation-free analysis, and selectivity through the use of MIPs as a synthetic, more stable, cheaper and easily prepared alternative to bio-macromolecules.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    10406 - Analytical chemistry

Návaznosti výsledku

  • Projekt

    Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

  • Návaznosti

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Ostatní

  • Rok uplatnění

    2021

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    Sensors and Actuators B: Chemical

  • ISSN

    0925-4005

  • e-ISSN

  • Svazek periodika

    341

  • Číslo periodika v rámci svazku

    129999

  • Stát vydavatele periodika

    CH - Švýcarská konfederace

  • Počet stran výsledku

    10

  • Strana od-do

    1-10

  • Kód UT WoS článku

    000652632200001

  • EID výsledku v databázi Scopus