Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43919859" target="_blank" >RIV/62156489:43210/21:43919859 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26620/21:PU140946

Výsledek na webu

<a href="https://doi.org/10.1016/j.snb.2021.129999" target="_blank" >https://doi.org/10.1016/j.snb.2021.129999</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2021.129999" target="_blank" >10.1016/j.snb.2021.129999</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers

Popis výsledku v původním jazyce

Microfluidic paper-based analytical devices (μPADs) offer the advantages of simplicity, extremely low costs, robustness, and miniaturisation, synergistically supporting portability and point-of-care (POC) analysis. When μPADs are combined with distance-based detection in DμPADs, they uniquely enable a quantitative analytical platform that is truly instrumentation-free (naked-eye readout), or at least, does not require any specialised scientific instrumentation (only mobile phone camera). However, a significant drawback of DμPADs is their limited selectivity. In this work, we present for the first time molecularly imprinted polymer (MIP) as a selectivity-enhancing element in MIP-modified DμPADs (MIP-DμPADs). Herein, a layer of polydopamine MIP was coated onto the paper substrate of a DμPAD, in a simple process using dopamine as the monomer deposited onto the paper matrix in the migration-detection zone of the DμPAD, and polymerised in a rapid low cost procedure in the presence of oxygen under alkaline conditions. The polydopamine MIP-DμPAD was then systematically investigated for the selective determination of chymotrypsinogen (chymo) as a model protein biomarker in urine, within the linear concentration range 2.4-29.2 μM (R2 = 0,9903) with corresponding relative standard deviations ranging from 2% to 11 % and LOD =3.5 μM and LOQ =11.8 μM. The here presented analytical concept based on MIP-DμPADs has a potential in POC diagnostics, because of the combination of low cost automated fabrication, the rapid quantitative near to instrumentation-free analysis, and selectivity through the use of MIPs as a synthetic, more stable, cheaper and easily prepared alternative to bio-macromolecules.

Název v anglickém jazyce

Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers

Popis výsledku anglicky

Microfluidic paper-based analytical devices (μPADs) offer the advantages of simplicity, extremely low costs, robustness, and miniaturisation, synergistically supporting portability and point-of-care (POC) analysis. When μPADs are combined with distance-based detection in DμPADs, they uniquely enable a quantitative analytical platform that is truly instrumentation-free (naked-eye readout), or at least, does not require any specialised scientific instrumentation (only mobile phone camera). However, a significant drawback of DμPADs is their limited selectivity. In this work, we present for the first time molecularly imprinted polymer (MIP) as a selectivity-enhancing element in MIP-modified DμPADs (MIP-DμPADs). Herein, a layer of polydopamine MIP was coated onto the paper substrate of a DμPAD, in a simple process using dopamine as the monomer deposited onto the paper matrix in the migration-detection zone of the DμPAD, and polymerised in a rapid low cost procedure in the presence of oxygen under alkaline conditions. The polydopamine MIP-DμPAD was then systematically investigated for the selective determination of chymotrypsinogen (chymo) as a model protein biomarker in urine, within the linear concentration range 2.4-29.2 μM (R2 = 0,9903) with corresponding relative standard deviations ranging from 2% to 11 % and LOD =3.5 μM and LOQ =11.8 μM. The here presented analytical concept based on MIP-DμPADs has a potential in POC diagnostics, because of the combination of low cost automated fabrication, the rapid quantitative near to instrumentation-free analysis, and selectivity through the use of MIPs as a synthetic, more stable, cheaper and easily prepared alternative to bio-macromolecules.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors and Actuators B. Chemical

ISSN

0925-4005

e-ISSN

—

Svazek periodika

341

Číslo periodika v rámci svazku

15 August

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

129999

Kód UT WoS článku

000652632200001

EID výsledku v databázi Scopus

2-s2.0-85105897263