Metallothionein sensing using molecularly imprinted polymers coupled laser ablation inductively coupled plasma-mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F21%3APU141184" target="_blank" >RIV/00216305:26620/21:PU141184 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.confer.cz/nanocon/2020/3749-mettalothionein-sensing-using-molecularly-imprinted-polymers-coupled-to-laser-ablation-inductively-coupled-plasma-mass-spectrometry" target="_blank" >https://www.confer.cz/nanocon/2020/3749-mettalothionein-sensing-using-molecularly-imprinted-polymers-coupled-to-laser-ablation-inductively-coupled-plasma-mass-spectrometry</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2020.3749" target="_blank" >10.37904/nanocon.2020.3749</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Metallothionein sensing using molecularly imprinted polymers coupled laser ablation inductively coupled plasma-mass spectrometry

Popis výsledku v původním jazyce

In this work, we report a facile method for a detection of mettalothionein (MT) - potential biomarker of tumor diseases. Combination of laser ablation inductively coupled plasma coupled to mass spectrometric detection (LA-ICP-MS) with sample pretreatment by magnetic particles modified by molecularly imprinted polymers (Mag-MIP) enables selective and sensitive protein detection and quantification. After Mag-MIP MT extraction, elements specific for the protein (S, Zn and Cd) were detected in healthy pig skin and pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. It was found that levels of detected elements were significantly elevated in melanoma tissue compared to healthy skin. Currently, the investigation of MT dimerization in cancer progression is carried out taking advantage from easily controllable in vitro MT dimerization which is used for simple preparation of selective MIP extraction phase. Unlike other recognition elements, which are not always available for target analyte (e.g. antibodies), MIPs can be easily prepared as required. Moreover, magnetic particles as a MIP substrate enable elegant extraction approach and LA-ICP-MS provides extremely sensitive detection. Therefore, Mag-MIP-LA-ICP-MS is a unique cross-border combination of molecular and elemental analysis.

Název v anglickém jazyce

Metallothionein sensing using molecularly imprinted polymers coupled laser ablation inductively coupled plasma-mass spectrometry

Popis výsledku anglicky

In this work, we report a facile method for a detection of mettalothionein (MT) - potential biomarker of tumor diseases. Combination of laser ablation inductively coupled plasma coupled to mass spectrometric detection (LA-ICP-MS) with sample pretreatment by magnetic particles modified by molecularly imprinted polymers (Mag-MIP) enables selective and sensitive protein detection and quantification. After Mag-MIP MT extraction, elements specific for the protein (S, Zn and Cd) were detected in healthy pig skin and pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. It was found that levels of detected elements were significantly elevated in melanoma tissue compared to healthy skin. Currently, the investigation of MT dimerization in cancer progression is carried out taking advantage from easily controllable in vitro MT dimerization which is used for simple preparation of selective MIP extraction phase. Unlike other recognition elements, which are not always available for target analyte (e.g. antibodies), MIPs can be easily prepared as required. Moreover, magnetic particles as a MIP substrate enable elegant extraction approach and LA-ICP-MS provides extremely sensitive detection. Therefore, Mag-MIP-LA-ICP-MS is a unique cross-border combination of molecular and elemental analysis.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON 2020 CONFERENCE PROCEEDINGS

ISBN

978-80-87294-98-7

ISSN

2694-930X

e-ISSN

—

Počet stran výsledku

5

Strana od-do

385-389

Název nakladatele

Tanger

Místo vydání

neuveden

Místo konání akce

Brno, Hotel Voroněž

Datum konání akce

21. 10. 2020

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000664505500065