Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F23%3A00574401" target="_blank" >RIV/67985904:_____/23:00574401 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/23:00132618 RIV/62156489:43210/23:43923773

Výsledek na webu

<a href="https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=5452124316" target="_blank" >https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=5452124316</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d3ja00065f" target="_blank" >10.1039/d3ja00065f</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer

Popis výsledku v původním jazyce

Effective detection and sensitive quantification of disease markers enable a better understanding of processes leading to disease development. Therefore, we present an approach that combines the excellent selective recognition capabilities of molecularly imprinted polymers (MIPs) and sensitive elemental detection using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Dopamine was used as a functional monomer, and metallothionein (MT), a metal-containing protein (Zn and Cd), was the targeted tumor marker. Iron oxide magnetic particles were exploited as a carrier of the polymeric layer. Due to the agglomeration of the particles in the presence of an external magnet, the prepared molecularly imprinted polymers (Mag-MIPs(MT)) were capable of isolating MT molecules from a tissue sample. A sandwich immunoassay with antibodies labeled by using silver nanoparticles was used to confirm the binding of MT to Mag-MIPs(MT). The calculated efficiency of isolation from a standard MT solution (73.5 & mu,M) was 63.3% (46.5 & mu,M). Casein was used to verify the selectivity of the prepared Mag-MIPs. The prepared particles were used for sensitive quantification of MT in melanoma and healthy skin of the melanoma-bearing Libechov minipig (MeLiM), which confirms that this method is applicable for the investigation of the tumor microenvironment. We found a significantly higher content of Zn in tumor tissue than in healthy skin.

Název v anglickém jazyce

Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer

Popis výsledku anglicky

Effective detection and sensitive quantification of disease markers enable a better understanding of processes leading to disease development. Therefore, we present an approach that combines the excellent selective recognition capabilities of molecularly imprinted polymers (MIPs) and sensitive elemental detection using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Dopamine was used as a functional monomer, and metallothionein (MT), a metal-containing protein (Zn and Cd), was the targeted tumor marker. Iron oxide magnetic particles were exploited as a carrier of the polymeric layer. Due to the agglomeration of the particles in the presence of an external magnet, the prepared molecularly imprinted polymers (Mag-MIPs(MT)) were capable of isolating MT molecules from a tissue sample. A sandwich immunoassay with antibodies labeled by using silver nanoparticles was used to confirm the binding of MT to Mag-MIPs(MT). The calculated efficiency of isolation from a standard MT solution (73.5 & mu,M) was 63.3% (46.5 & mu,M). Casein was used to verify the selectivity of the prepared Mag-MIPs. The prepared particles were used for sensitive quantification of MT in melanoma and healthy skin of the melanoma-bearing Libechov minipig (MeLiM), which confirms that this method is applicable for the investigation of the tumor microenvironment. We found a significantly higher content of Zn in tumor tissue than in healthy skin.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Analytical Atomic Spectrometry

ISSN

0267-9477

e-ISSN

1364-5544

Svazek periodika

38

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

1618-1625

Kód UT WoS článku

001022916300001

EID výsledku v databázi Scopus

2-s2.0-85165440787