The optimization of microchip digital polymerase chain reaction (dPCR) with microwell sample dispersion

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F21%3APU142850" target="_blank" >RIV/00216305:26620/21:PU142850 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The optimization of microchip digital polymerase chain reaction (dPCR) with microwell sample dispersion

Popis výsledku v původním jazyce

The digital polymerase chain reaction (dPCR) rising in development and popularity. dPCR is a suitable solution for the analysis of rare samples or samples available only in small quantities. The determination of absolute copy number of target DNA without the use of external standards together with the possibility of statistical analysis due to the binary nature of the data are the main advantages of dPCR. Chip dPCR compared with droplet dPCR is less widely used and developed. The precision and dynamic range of dPCR is dependent on the number of reaction wells. The increment of the number of reaction wells on chip, leads to the issue of appropriate method of sample loading. Our designed and fabricated silicon chips with 26 448 microwells with the volume of one microwell 59 pL was tested for suitable method of sampling for our experimental dPCR technology. During incorrect sample loading on chip surface, it arises to the amplification defects e.g. evaporation of sample, cluster formation, uneven fluores

Název v anglickém jazyce

The optimization of microchip digital polymerase chain reaction (dPCR) with microwell sample dispersion

Popis výsledku anglicky

The digital polymerase chain reaction (dPCR) rising in development and popularity. dPCR is a suitable solution for the analysis of rare samples or samples available only in small quantities. The determination of absolute copy number of target DNA without the use of external standards together with the possibility of statistical analysis due to the binary nature of the data are the main advantages of dPCR. Chip dPCR compared with droplet dPCR is less widely used and developed. The precision and dynamic range of dPCR is dependent on the number of reaction wells. The increment of the number of reaction wells on chip, leads to the issue of appropriate method of sample loading. Our designed and fabricated silicon chips with 26 448 microwells with the volume of one microwell 59 pL was tested for suitable method of sampling for our experimental dPCR technology. During incorrect sample loading on chip surface, it arises to the amplification defects e.g. evaporation of sample, cluster formation, uneven fluores

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Projekt

<a href="/cs/project/LM2018110" target="_blank" >LM2018110: Výzkumná infrastruktura CzechNanoLab</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů