Electrophysiological investigation of intact retina with soft printed organic neural interface

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F21%3APU143436" target="_blank" >RIV/00216305:26620/21:PU143436 - isvavai.cz</a>

Výsledek na webu

<a href="https://iopscience.iop.org/article/10.1088/1741-2552/ac36ab" target="_blank" >https://iopscience.iop.org/article/10.1088/1741-2552/ac36ab</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1741-2552/ac36ab" target="_blank" >10.1088/1741-2552/ac36ab</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrophysiological investigation of intact retina with soft printed organic neural interface

Popis výsledku v původním jazyce

Objective. Understanding how the retina converts a natural image or an electrically stimulated one into neural firing patterns is the focus of on-going research activities. Ex vivo, the retina can be readily investigated using multi electrode arrays (MEAs). However, MEA recording and stimulation from an intact retina (in the eye) has been so far insufficient. Approach. In the present study, we report new soft carbon electrode arrays suitable for recording and stimulating neural activity in an intact retina. Screen-printing of carbon ink on 20 mu m polyurethane (PU) film was used to realize electrode arrays with electrodes as small as 40 mu m in diameter. Passivation was achieved with a holey membrane, realized using laser drilling in a thin (50 mu m) PU film. Plasma polymerized 3.4-ethylenedioxythiophene was used to coat the electrode array to improve the electrode specific capacitance. Chick retinas, embryonic stage day 13, both explanted and intact inside an enucleated eye, were used. Main results. A novel fabrication process based on printed carbon electrodes was developed and yielded high capacitance electrodes on a soft substrate. Ex vivo electrical recording of retina activity with carbon electrodes is demonstrated. With the addition of organic photo-capacitors, simultaneous photo-electrical stimulation and electrical recording was achieved. Finally, electrical activity recordings from an intact chick retina (inside enucleated eyes) were demonstrated. Both photosensitive retinal ganglion cell responses and spontaneous retina waves were recorded and their features analyzed. Significance. Results of this study demonstrated soft electrode arrays with unique properties, suitable for simultaneous recording and photo-electrical stimulation of the retina at high fidelity. This novel electrode technology opens up new frontiers in the study of neural tissue in vivo.

Název v anglickém jazyce

Electrophysiological investigation of intact retina with soft printed organic neural interface

Popis výsledku anglicky

Objective. Understanding how the retina converts a natural image or an electrically stimulated one into neural firing patterns is the focus of on-going research activities. Ex vivo, the retina can be readily investigated using multi electrode arrays (MEAs). However, MEA recording and stimulation from an intact retina (in the eye) has been so far insufficient. Approach. In the present study, we report new soft carbon electrode arrays suitable for recording and stimulating neural activity in an intact retina. Screen-printing of carbon ink on 20 mu m polyurethane (PU) film was used to realize electrode arrays with electrodes as small as 40 mu m in diameter. Passivation was achieved with a holey membrane, realized using laser drilling in a thin (50 mu m) PU film. Plasma polymerized 3.4-ethylenedioxythiophene was used to coat the electrode array to improve the electrode specific capacitance. Chick retinas, embryonic stage day 13, both explanted and intact inside an enucleated eye, were used. Main results. A novel fabrication process based on printed carbon electrodes was developed and yielded high capacitance electrodes on a soft substrate. Ex vivo electrical recording of retina activity with carbon electrodes is demonstrated. With the addition of organic photo-capacitors, simultaneous photo-electrical stimulation and electrical recording was achieved. Finally, electrical activity recordings from an intact chick retina (inside enucleated eyes) were demonstrated. Both photosensitive retinal ganglion cell responses and spontaneous retina waves were recorded and their features analyzed. Significance. Results of this study demonstrated soft electrode arrays with unique properties, suitable for simultaneous recording and photo-electrical stimulation of the retina at high fidelity. This novel electrode technology opens up new frontiers in the study of neural tissue in vivo.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10610 - Biophysics

Projekt

—

Návaznosti

R - Projekt Ramcoveho programu EK

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Neural Engineering

ISSN

1741-2560

e-ISSN

1741-2552

Svazek periodika

18

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

„066017-1“-„066017-12“

Kód UT WoS článku

000720536400001

EID výsledku v databázi Scopus

2-s2.0-85120820493