An enzyme cascade biosensor based on multiwalled carbon nanotube-RuO2 nanocomposite for selective amperometric determination of lactose in milk samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F24%3APU152388" target="_blank" >RIV/00216305:26620/24:PU152388 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985882:_____/24:00588275 RIV/00216224:14740/24:00138500 RIV/62156489:43210/24:43925374

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0026265X24012505?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0026265X24012505?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2024.111138" target="_blank" >10.1016/j.microc.2024.111138</a>

Jazyk výsledku

angličtina

Název v původním jazyce

An enzyme cascade biosensor based on multiwalled carbon nanotube-RuO2 nanocomposite for selective amperometric determination of lactose in milk samples

Popis výsledku v původním jazyce

An enzyme-based electrochemical biosensor was fabricated for the sensitive determination of lactose. The utilized enzyme cascade system is composed of 8-galactosidase (8-Gal) and glucose oxidase (GOx). The ruthenium (IV) oxide (RuO2) presents in MWCNT-RuO2 nanocomposite immobilized on the glassy carbon electrode acts as an electrochemical mediator, resembling a second-generation enzyme biosensor. The functional mechanism of the biosensor was discussed, explaining the chemical oxidation of H2O2, the final product of the enzymatic reaction, by RuO2 and subsequent reoxidation of generated Ru to RuO2 at the electrode surface. This shifts the oxidation of H2O2 to a lower potential magnitude of +0.40 V, enhancing the biosensor's selectivity. The analytical figures of merit were verified for the developed lactose biosensor through repetitive measurements. The precision of the lactose biosensor was ensured with good reproducibility (RSD % = 2.68) and repeatability (RSD % = 4.12). The selectivity of the biosensor towards various saccharides and ionic species potentially present in milk samples was investigated, and no notable interference effect was detected. Moreover, the accuracy of the lactose biosensor was tested by analyzing spiked samples and a semi-skimmed milk (SS-milk) sample with certified lactose values. The limit of detection (LOD) and limit of quantification (LOQ) were calculated to be (0.036 mM) and (0.121 mM), respectively. Thanks to the short response time of the fabricated lactose biosensor, it was transferred to a screen-printed carbon electrode (SPCE) and successfully employed in flow injection analysis (FIA).

Název v anglickém jazyce

An enzyme cascade biosensor based on multiwalled carbon nanotube-RuO2 nanocomposite for selective amperometric determination of lactose in milk samples

Popis výsledku anglicky

An enzyme-based electrochemical biosensor was fabricated for the sensitive determination of lactose. The utilized enzyme cascade system is composed of 8-galactosidase (8-Gal) and glucose oxidase (GOx). The ruthenium (IV) oxide (RuO2) presents in MWCNT-RuO2 nanocomposite immobilized on the glassy carbon electrode acts as an electrochemical mediator, resembling a second-generation enzyme biosensor. The functional mechanism of the biosensor was discussed, explaining the chemical oxidation of H2O2, the final product of the enzymatic reaction, by RuO2 and subsequent reoxidation of generated Ru to RuO2 at the electrode surface. This shifts the oxidation of H2O2 to a lower potential magnitude of +0.40 V, enhancing the biosensor's selectivity. The analytical figures of merit were verified for the developed lactose biosensor through repetitive measurements. The precision of the lactose biosensor was ensured with good reproducibility (RSD % = 2.68) and repeatability (RSD % = 4.12). The selectivity of the biosensor towards various saccharides and ionic species potentially present in milk samples was investigated, and no notable interference effect was detected. Moreover, the accuracy of the lactose biosensor was tested by analyzing spiked samples and a semi-skimmed milk (SS-milk) sample with certified lactose values. The limit of detection (LOD) and limit of quantification (LOQ) were calculated to be (0.036 mM) and (0.121 mM), respectively. Thanks to the short response time of the fabricated lactose biosensor, it was transferred to a screen-printed carbon electrode (SPCE) and successfully employed in flow injection analysis (FIA).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/EF18_046%2F0015974" target="_blank" >EF18_046/0015974: Modernizace České infrastruktury pro integrativní strukturní biologii</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MICROCHEMICAL JOURNAL

ISSN

0026-265X

e-ISSN

1095-9149

Svazek periodika

204

Číslo periodika v rámci svazku

111138

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

12

Strana od-do

„“-„“

Kód UT WoS článku

001272028000001

EID výsledku v databázi Scopus

2-s2.0-85198242094