Immunochemical Applications Utilizing Laser-Induced Breakdown Spectroscopy Detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F26%3A0199443" target="_blank" >RIV/00216305:26620/26:0199443 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/25:00144353

Výsledek na webu

<a href="https://link.springer.com/chapter/10.1007/978-3-031-85975-5_11" target="_blank" >https://link.springer.com/chapter/10.1007/978-3-031-85975-5_11</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-3-031-85975-5_11" target="_blank" >10.1007/978-3-031-85975-5_11</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immunochemical Applications Utilizing Laser-Induced Breakdown Spectroscopy Detection

Popis výsledku v původním jazyce

Immunochemistry specifically detects or visualizes biologically important analytes, such as disease biomarkers or pathogens, employing various detection techniques. This can be done by employing label-based assays for indirect detection of the analytes. The labels for immunochemical methods, typically with luminescence or enzymatic properties, are selected to allow a simple and fast detection, high accuracy, and reproducibility of the measurements. In this chapter, immunochemical methodology based on laser-induced breakdown spectroscopy (LIBS) detection is mainly discussed in comparison with other commonly used techniques based on luminescence or color change monitoring. Being a multielemental analytical technique, LIBS allows for the differentiation of the labels based on their elemental composition with no need for specific label properties (e.g., luminescence) while maintaining the information on the distribution of other valuable constituents. For the sake of complementarity, laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) as a detection technique for immunochemical methods is also included in the chapter as it serves not only as a reference for the tag-LIBS technique. With the continual advancement of the instrumentation of both techniques, the possibility of a tandem analysis by tag-LIBS and LA-ICP-MS might be explored in the future.

Název v anglickém jazyce

Immunochemical Applications Utilizing Laser-Induced Breakdown Spectroscopy Detection

Popis výsledku anglicky

Immunochemistry specifically detects or visualizes biologically important analytes, such as disease biomarkers or pathogens, employing various detection techniques. This can be done by employing label-based assays for indirect detection of the analytes. The labels for immunochemical methods, typically with luminescence or enzymatic properties, are selected to allow a simple and fast detection, high accuracy, and reproducibility of the measurements. In this chapter, immunochemical methodology based on laser-induced breakdown spectroscopy (LIBS) detection is mainly discussed in comparison with other commonly used techniques based on luminescence or color change monitoring. Being a multielemental analytical technique, LIBS allows for the differentiation of the labels based on their elemental composition with no need for specific label properties (e.g., luminescence) while maintaining the information on the distribution of other valuable constituents. For the sake of complementarity, laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) as a detection technique for immunochemical methods is also included in the chapter as it serves not only as a reference for the tag-LIBS technique. With the continual advancement of the instrumentation of both techniques, the possibility of a tandem analysis by tag-LIBS and LA-ICP-MS might be explored in the future.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA22-27580S" target="_blank" >GA22-27580S: Laserová spektroskopie v imunostanovení a zobrazování s nanometalickými značkami</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Laser-Induced Breakdown Spectroscopy in Biological, Forensic and Materials Sciences

ISBN

9783031859748

Počet stran výsledku

26

Strana od-do

331-356

Počet stran knihy

559

Název nakladatele

Springer Nature Switzerland

Místo vydání

Cham

Kód UT WoS kapitoly

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