CRISPR/Cas9-Assisted Microrobots for Fast and Ultrasensitive "On-The-Fly" Next-Generation DNA Detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F26%3A0199447" target="_blank" >RIV/00216305:26620/26:0199447 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989100:27240/25:10258518

Výsledek na webu

<a href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/adfm.202510978" target="_blank" >https://advanced.onlinelibrary.wiley.com/doi/10.1002/adfm.202510978</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/adfm.202510978" target="_blank" >10.1002/adfm.202510978</a>

Jazyk výsledku

angličtina

Název v původním jazyce

CRISPR/Cas9-Assisted Microrobots for Fast and Ultrasensitive "On-The-Fly" Next-Generation DNA Detection

Popis výsledku v původním jazyce

Fast, sensitive, and selective nucleic acids detection is essential for applications in clinical diagnostics, food safety, and environmental monitoring. Hence, in this study, we introduce a novel motion-assisted CRISPR/Cas9 microrobots-based biosensor, consisting of CRISPR/Cas9 functionalized on gold microrobots (Au-MRs) forming CRISPR/Cas9@Au-MRs, which exhibit self-propulsion leading to rapid and ultrasensitive detection of target DNA. The proposed system achieved the limit of detection in low fM DNA concentration, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any previously reported systems. Further, this self-propelled system removes the need for complicated sample preparation, enabling real-time detection by using a minimal amount of sample. Unlike conventional CRISPR biosensors, this platform utilizes "on-the-fly" motion-enhanced signal recovery, ensuring superior specificity and sensitivity in diverse biological environments. Importantly, the presented system shows exceptional analytical performance in biological samples, highlighting its potential for real-world sensing applications. Overall, the motion-assisted CRISPR/Cas9@Au-MRs biosensor represents evolutionary progress in nucleic acids detection, providing a fast, reliable platform that can be easily scalable for applications in disease detection, biosafety and environmental detection.

Název v anglickém jazyce

CRISPR/Cas9-Assisted Microrobots for Fast and Ultrasensitive "On-The-Fly" Next-Generation DNA Detection

Popis výsledku anglicky

Fast, sensitive, and selective nucleic acids detection is essential for applications in clinical diagnostics, food safety, and environmental monitoring. Hence, in this study, we introduce a novel motion-assisted CRISPR/Cas9 microrobots-based biosensor, consisting of CRISPR/Cas9 functionalized on gold microrobots (Au-MRs) forming CRISPR/Cas9@Au-MRs, which exhibit self-propulsion leading to rapid and ultrasensitive detection of target DNA. The proposed system achieved the limit of detection in low fM DNA concentration, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any previously reported systems. Further, this self-propelled system removes the need for complicated sample preparation, enabling real-time detection by using a minimal amount of sample. Unlike conventional CRISPR biosensors, this platform utilizes "on-the-fly" motion-enhanced signal recovery, ensuring superior specificity and sensitivity in diverse biological environments. Importantly, the presented system shows exceptional analytical performance in biological samples, highlighting its potential for real-world sensing applications. Overall, the motion-assisted CRISPR/Cas9@Au-MRs biosensor represents evolutionary progress in nucleic acids detection, providing a fast, reliable platform that can be easily scalable for applications in disease detection, biosafety and environmental detection.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21000 - Nano-technology

Projekt

<a href="/cs/project/GX25-15484X" target="_blank" >GX25-15484X: Inteligentní mikro- a nanoroboti pro čištění vody</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2026

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Advanced functional materials

ISSN

1616-301X

e-ISSN

1616-3028

Svazek periodika

36

Číslo periodika v rámci svazku

14

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

12

Strana od-do

—

Kód UT WoS článku

001584845900001

EID výsledku v databázi Scopus

2-s2.0-105018211721