Comparison of two methods for dimethylarginines quantification

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00669806%3A_____%2F24%3A10477054" target="_blank" >RIV/00669806:_____/24:10477054 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/24:10477054

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=YnesWtWrin" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=YnesWtWrin</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plabm.2024.e00359" target="_blank" >10.1016/j.plabm.2024.e00359</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of two methods for dimethylarginines quantification

Popis výsledku v původním jazyce

Objectives: Both dimethylarginines are widely bound to chronic kidney disease (CKD). This study was focused to validate published LC-MS/MS method and compared the measured data with an immunoassay. Design and methods: The analysis was performed on a Dionex UltiMate 3000 UHPLC-Standard (Thermo Fisher Scientific, Waltham, Massachusetts, USA) with an amaZon SL ion trap (Bruker, Billerica, Massachusetts, USA). Comparison was evaluated by using Passing Bablok regression and Bland Altman plot. Healthy volunteers (n = 40) were used for validation and as control group to patients group (n = 40) with different stages of CKD. Results: The results in healthy controls determined by the LC-MS/MS (ELISA) method were 0.52 +- 0.0892 with 95 % CI: 0.49-0.55 (0.61 +- 0.1213 with 95 % CI: 0.57-0.64) μmol/L for AD MA and 0.56 +- 0.0810 with 95 % CI: 0.53-0.58 (0.62 +- 0.0752 with 95 % CI: 0.57-0.65) μmol/L for SDMA. In the same way, the patient group values determined by the LC-MS/MS (ELISA) method were 0.82 +- 0.1604 with 95 % CI: 0.75-0.88 (1.06 +- 0.3002 with 95 % CI: 0.94-1.19) μmol/L and 2.14 +- 0.8778 with 95 % CI: 1.47-2.58 (1.65 +- 0.5160 with 95 % CI: 1.40-1.98) μmol/L for ADMA and SDMA, respectively. The correlation between the methods, expressed as the Spearman correlation coefficient (R), was 0.858 (0.8059) for ADMA (p &lt; 0.0001) and 0.895 (0.9607) for SDMA (p &lt; 0.0001). Conclusions: ADMA levels determined by the immunoassay were almost 30 % overestimated, in contrast to SDMA levels, which were 3 % underestimated. According to our findings, a better correlation could be obtained by simple sample dilution.

Název v anglickém jazyce

Comparison of two methods for dimethylarginines quantification

Popis výsledku anglicky

Objectives: Both dimethylarginines are widely bound to chronic kidney disease (CKD). This study was focused to validate published LC-MS/MS method and compared the measured data with an immunoassay. Design and methods: The analysis was performed on a Dionex UltiMate 3000 UHPLC-Standard (Thermo Fisher Scientific, Waltham, Massachusetts, USA) with an amaZon SL ion trap (Bruker, Billerica, Massachusetts, USA). Comparison was evaluated by using Passing Bablok regression and Bland Altman plot. Healthy volunteers (n = 40) were used for validation and as control group to patients group (n = 40) with different stages of CKD. Results: The results in healthy controls determined by the LC-MS/MS (ELISA) method were 0.52 +- 0.0892 with 95 % CI: 0.49-0.55 (0.61 +- 0.1213 with 95 % CI: 0.57-0.64) μmol/L for AD MA and 0.56 +- 0.0810 with 95 % CI: 0.53-0.58 (0.62 +- 0.0752 with 95 % CI: 0.57-0.65) μmol/L for SDMA. In the same way, the patient group values determined by the LC-MS/MS (ELISA) method were 0.82 +- 0.1604 with 95 % CI: 0.75-0.88 (1.06 +- 0.3002 with 95 % CI: 0.94-1.19) μmol/L and 2.14 +- 0.8778 with 95 % CI: 1.47-2.58 (1.65 +- 0.5160 with 95 % CI: 1.40-1.98) μmol/L for ADMA and SDMA, respectively. The correlation between the methods, expressed as the Spearman correlation coefficient (R), was 0.858 (0.8059) for ADMA (p &lt; 0.0001) and 0.895 (0.9607) for SDMA (p &lt; 0.0001). Conclusions: ADMA levels determined by the immunoassay were almost 30 % overestimated, in contrast to SDMA levels, which were 3 % underestimated. According to our findings, a better correlation could be obtained by simple sample dilution.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30100 - Basic medicine

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Practical Laboratory Medicine

ISSN

2352-5517

e-ISSN

2352-5517

Svazek periodika

39

Číslo periodika v rámci svazku

March

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

e00359

Kód UT WoS článku

001175273100001

EID výsledku v databázi Scopus

2-s2.0-85183763540