Correlation study of enzyme-linked immunosorbent assay and high-performance liquid chromatography/tandem mass spectrometry method for the determination of N-methylcarbamate insecticides in baby food

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F03%3A00008477" target="_blank" >RIV/60461373:22330/03:00008477 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Correlation study of enzyme-linked immunosorbent assay and high-performance liquid chromatography/tandem mass spectrometry method for the determination of N-methylcarbamate insecticides in baby food

Popis výsledku v původním jazyce

In this work, a correlation study of monoclonal antibody-based enzyme-linked immunosorbent assays (ELISAs) and a liquid chromatography-electrospray mass spectrometric (HPLC/ESI/MS/MS) method for the determination of N-methyl carbamate insecticides carbofuran, carbaryl and methiocarb in fruit baby food is presented. The comparison of performance characteristics of the two methods was carried out by simultaneous analysis of apple-strawberry baby food (GPC purified) extracts spiked with N-methylcarbamatesat six different concentration levels. Results obtained by ELISA correlated well with those obtained by LC/MS/MS, both in terms of trueness and precision. Recoveries, i.e. the ratio of the determined concentration to the known spiked concentration, werein the 60-100% range for ELISA and in the 73-104 % range by LC/MS/MS with the RSDs from 7 replicate analyses 3.6 - 23.3 % and 1.7-8.2 %, respectively. The influence of sample pre-treatment on the analytical performance of immunoassay meth

Název v anglickém jazyce

Correlation study of enzyme-linked immunosorbent assay and high-performance liquid chromatography/tandem mass spectrometry method for the determination of N-methylcarbamate insecticides in baby food

Popis výsledku anglicky

In this work, a correlation study of monoclonal antibody-based enzyme-linked immunosorbent assays (ELISAs) and a liquid chromatography-electrospray mass spectrometric (HPLC/ESI/MS/MS) method for the determination of N-methyl carbamate insecticides carbofuran, carbaryl and methiocarb in fruit baby food is presented. The comparison of performance characteristics of the two methods was carried out by simultaneous analysis of apple-strawberry baby food (GPC purified) extracts spiked with N-methylcarbamatesat six different concentration levels. Results obtained by ELISA correlated well with those obtained by LC/MS/MS, both in terms of trueness and precision. Recoveries, i.e. the ratio of the determined concentration to the known spiked concentration, werein the 60-100% range for ELISA and in the 73-104 % range by LC/MS/MS with the RSDs from 7 replicate analyses 3.6 - 23.3 % and 1.7-8.2 %, respectively. The influence of sample pre-treatment on the analytical performance of immunoassay meth

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Anal.Chim.Acta

ISSN

0003-2670

e-ISSN

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Svazek periodika

495

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

123-132

Kód UT WoS článku

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EID výsledku v databázi Scopus

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