Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite antibodies. comparison with the free light chain oligoclonal pattern

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F12%3A00102563" target="_blank" >RIV/00843989:_____/12:00102563 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1186/2045-8118-9-5" target="_blank" >http://dx.doi.org/10.1186/2045-8118-9-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/2045-8118-9-5" target="_blank" >10.1186/2045-8118-9-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite antibodies. comparison with the free light chain oligoclonal pattern

Popis výsledku v původním jazyce

Oligoclonal IgG bands in cerebrospinal fluid that are absent in serum indicate intrathecal IgG synthesis and are a sensitive marker of CNS inflammatory diseases, in particular multiple sclerosis. It may be of interest to determine whether these bands arepredominantly IgG? or IgG?. We have used Hevylite antibodies and developed a technique for detection of oligoclonal IgG? and IgG? bands by means of isoelectric focusing followed by immunoblotting. The same technique was used for oligoclonal free ? and free ? detection. Among several techniques tested, affinity immunoblotting appears to be the most sensitive; it can detect less than 1 ng of IgG? or IgG? paraprotein. We compared oligoclonal IgG profiles with those of oligoclonal IgG? and IgG?. There wasgood agreement concerning the presence or absence of intrathecal synthesis. We observed the ratios between oligoclonal IgG? and IgG? bands, and they did not always match the ratios between free ? and free ? bands. We were also able to det

Název v anglickém jazyce

Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite antibodies. comparison with the free light chain oligoclonal pattern

Popis výsledku anglicky

Oligoclonal IgG bands in cerebrospinal fluid that are absent in serum indicate intrathecal IgG synthesis and are a sensitive marker of CNS inflammatory diseases, in particular multiple sclerosis. It may be of interest to determine whether these bands arepredominantly IgG? or IgG?. We have used Hevylite antibodies and developed a technique for detection of oligoclonal IgG? and IgG? bands by means of isoelectric focusing followed by immunoblotting. The same technique was used for oligoclonal free ? and free ? detection. Among several techniques tested, affinity immunoblotting appears to be the most sensitive; it can detect less than 1 ng of IgG? or IgG? paraprotein. We compared oligoclonal IgG profiles with those of oligoclonal IgG? and IgG?. There wasgood agreement concerning the presence or absence of intrathecal synthesis. We observed the ratios between oligoclonal IgG? and IgG? bands, and they did not always match the ratios between free ? and free ? bands. We were also able to det

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Fluids and barriers of the CNS [online]

ISSN

2045-8118

e-ISSN

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Svazek periodika

9

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

5

Kód UT WoS článku

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EID výsledku v databázi Scopus

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