Quantitation of IgG kappa and IgG lambda in the cerebrospinal fluid by sandwich ELISA method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F17%3AE0106373" target="_blank" >RIV/00843989:_____/17:E0106373 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1080/15321819.2016.1233889" target="_blank" >http://dx.doi.org/10.1080/15321819.2016.1233889</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/15321819.2016.1233889" target="_blank" >10.1080/15321819.2016.1233889</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Quantitation of IgG kappa and IgG lambda in the cerebrospinal fluid by sandwich ELISA method

Popis výsledku v původním jazyce

IgG kappa and IgG lambda concentrations were quantified in 96 paired CSF and sera using HevyliteTM antibodies in an in-house developed sandwich ELISA method. In 56 of these samples, the results were compared with a qualitative isoelectric focusing/affinity-mediated immunoblotting assay for oligoclonal IgG kappa and IgG lambda. Normal IgG kappa/lambda ratio in the CSF was the same as in serum. In 19/33 patients with intrathecal oligoclonal IgG synthesis, skewed IgG kappa/lambda ratio was observed (increased in 16 and decreased in 3 cases). The analysis of light chain composition of intrathecally synthesised immunoglobulins could contribute to our understanding of intrathecal humoral immune response, although its diagnostic utility is limited.

Název v anglickém jazyce

Quantitation of IgG kappa and IgG lambda in the cerebrospinal fluid by sandwich ELISA method

Popis výsledku anglicky

IgG kappa and IgG lambda concentrations were quantified in 96 paired CSF and sera using HevyliteTM antibodies in an in-house developed sandwich ELISA method. In 56 of these samples, the results were compared with a qualitative isoelectric focusing/affinity-mediated immunoblotting assay for oligoclonal IgG kappa and IgG lambda. Normal IgG kappa/lambda ratio in the CSF was the same as in serum. In 19/33 patients with intrathecal oligoclonal IgG synthesis, skewed IgG kappa/lambda ratio was observed (increased in 16 and decreased in 3 cases). The analysis of light chain composition of intrathecally synthesised immunoglobulins could contribute to our understanding of intrathecal humoral immune response, although its diagnostic utility is limited.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10609 - Biochemical research methods

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Immunoassay and Immunochemistry

ISSN

1532-1819

e-ISSN

1532-4230

Svazek periodika

38

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

165-177

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-84988926729