Surface plasmon resonance imaging - first experience

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00843989%3A_____%2F14%3AE0104495" target="_blank" >RIV/00843989:_____/14:E0104495 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Surface plasmon resonance imaging - first experience

Popis výsledku v původním jazyce

Aim of the study were to optimize the preparation of biochips, set up and launch a new SPRi-Lab+ ™ device and SPRi-Arrayer spotting equipment (by HORIBA company). For the given task, pilot method for the ovalbuminu (OVA) determination was chosen. Surface plasmon resonance imaging (SPRi) can be used for the determination of biomolecules on the surface of the chip (prism with nanolayer deposited gold) in real time, without labeling any of the reactants. Analysers applying this technique use CCD camera for sensing of the biochip surface (microscopic image of surface). The preparation of biochips by using the SPRi-Arrayer equipment and determination of ovalbumin were optimized. Analytical characteristics of this method were statistically established. The limit of detection (0.0041 mg.l-1) based on measurements of the blank, sensitivity of the method and detection limit of the calibration dependence were determined. Method for the determination of ovalbumin gives reliable results in a concentration ranging from 0.01 to 10.00 mg.l-1.

Název v anglickém jazyce

Surface plasmon resonance imaging - first experience

Popis výsledku anglicky

Aim of the study were to optimize the preparation of biochips, set up and launch a new SPRi-Lab+ ™ device and SPRi-Arrayer spotting equipment (by HORIBA company). For the given task, pilot method for the ovalbuminu (OVA) determination was chosen. Surface plasmon resonance imaging (SPRi) can be used for the determination of biomolecules on the surface of the chip (prism with nanolayer deposited gold) in real time, without labeling any of the reactants. Analysers applying this technique use CCD camera for sensing of the biochip surface (microscopic image of surface). The preparation of biochips by using the SPRi-Arrayer equipment and determination of ovalbumin were optimized. Analytical characteristics of this method were statistically established. The limit of detection (0.0041 mg.l-1) based on measurements of the blank, sensitivity of the method and detection limit of the calibration dependence were determined. Method for the determination of ovalbumin gives reliable results in a concentration ranging from 0.01 to 10.00 mg.l-1.

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

CECE 2014. 11th International Interdisciplinary Meeting on Bioanalysis. October 20-22, 2014

ISBN

978-80-904959-2-0

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

390-393

Název nakladatele

Institut of Analytical Chemistry ASCR

Místo vydání

Brno

Místo konání akce

Brno, Czech Republic

Datum konání akce

20. 10. 2014

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000354547400126