Dynamic Self-Assembly and Stimuli-Responsive Disassembly of Bioactive-Loaded Cubosomes in Biomimetic Media Traced by Real-Time Small-Angle X-ray Scattering and Cryogenic Transmission Electron Microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F10974938%3A_____%2F25%3A25_88_38" target="_blank" >RIV/10974938:_____/25:25_88_38 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1021/acsami.5c18735" target="_blank" >https://doi.org/10.1021/acsami.5c18735</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsami.5c18735" target="_blank" >10.1021/acsami.5c18735</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dynamic Self-Assembly and Stimuli-Responsive Disassembly of Bioactive-Loaded Cubosomes in Biomimetic Media Traced by Real-Time Small-Angle X-ray Scattering and Cryogenic Transmission Electron Microscopy

Popis výsledku v původním jazyce

Designing advanced functional materials capable of passing through complex biological environments requires a deep understanding of their dynamic structural behavior in situ. We investigate pH-responsive core–shell cubosomes for oral drug delivery applications. These nanoparticles comprise a lipid-based core of cubic Im3m liquid crystalline structure and are coated with a chitosan-N-arginine/alginate polyelectrolyte shell (PS). The cubosomes encapsulate varying concentrations (0–30% w/w) of Aloe vera-derived acemannan, an immunomodulatory macromolecular drug. Utilizing synchrotron small-angle X-ray scattering and cryogenic transmission electron microscopy, we performed an advanced spatiotemporal analysis, which focused on their nanoscale structural evolution under simulated gastric (pH 2.5) and intestinal (pH 7.4) conditions. The interactions with key individual gastrointestinal components, including mucins, pepsin, bile salts, and pancreatin, were systematically examined. Our results demonstrate that acemannan incorporation and environmental pH significantly modulate cubosome structure and heterogeneity (phase coexistence) during disassembly. The pH-responsive polyelectrolyte shell imparts notable structural stability against pepsin and mucins at pH 2.5, ensuring functional gastric protection. However, under intestinal conditions (pH 7.4), bile salt-mediated solubilization caused complete disassembly. Pancreatic lipase-induced digestion triggered a remarkable time-dependent phase transition from a cubic (Im3m) to an inverted hexagonal (HII) topology in PS-cubosomes containing 30% acemannan. A simulated duodenum mixture induced lamellar phases at pH 2.5 for acemannan-loaded systems but led to complete disassembly at pH 7.4, primarily driven by bile salts. Deconvoluting these structural responses over time provides crucial insights into their mechanistic nature. It clarifies pH-dependent stability and component-specific disassembly pathways. The achieved understanding is crucial for designing advanced stimuli-responsive lipid/biopolymer nanomaterials that facilitate efficient oral delivery.

Název v anglickém jazyce

Dynamic Self-Assembly and Stimuli-Responsive Disassembly of Bioactive-Loaded Cubosomes in Biomimetic Media Traced by Real-Time Small-Angle X-ray Scattering and Cryogenic Transmission Electron Microscopy

Popis výsledku anglicky

Designing advanced functional materials capable of passing through complex biological environments requires a deep understanding of their dynamic structural behavior in situ. We investigate pH-responsive core–shell cubosomes for oral drug delivery applications. These nanoparticles comprise a lipid-based core of cubic Im3m liquid crystalline structure and are coated with a chitosan-N-arginine/alginate polyelectrolyte shell (PS). The cubosomes encapsulate varying concentrations (0–30% w/w) of Aloe vera-derived acemannan, an immunomodulatory macromolecular drug. Utilizing synchrotron small-angle X-ray scattering and cryogenic transmission electron microscopy, we performed an advanced spatiotemporal analysis, which focused on their nanoscale structural evolution under simulated gastric (pH 2.5) and intestinal (pH 7.4) conditions. The interactions with key individual gastrointestinal components, including mucins, pepsin, bile salts, and pancreatin, were systematically examined. Our results demonstrate that acemannan incorporation and environmental pH significantly modulate cubosome structure and heterogeneity (phase coexistence) during disassembly. The pH-responsive polyelectrolyte shell imparts notable structural stability against pepsin and mucins at pH 2.5, ensuring functional gastric protection. However, under intestinal conditions (pH 7.4), bile salt-mediated solubilization caused complete disassembly. Pancreatic lipase-induced digestion triggered a remarkable time-dependent phase transition from a cubic (Im3m) to an inverted hexagonal (HII) topology in PS-cubosomes containing 30% acemannan. A simulated duodenum mixture induced lamellar phases at pH 2.5 for acemannan-loaded systems but led to complete disassembly at pH 7.4, primarily driven by bile salts. Deconvoluting these structural responses over time provides crucial insights into their mechanistic nature. It clarifies pH-dependent stability and component-specific disassembly pathways. The achieved understanding is crucial for designing advanced stimuli-responsive lipid/biopolymer nanomaterials that facilitate efficient oral delivery.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS Applied Materials & Interfaces, 2025, xxx, xxx-xxx

ISSN

1944-8244

e-ISSN

1944-8252

Svazek periodika

17

Číslo periodika v rámci svazku

51

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

16

Strana od-do

69118 - 69133

Kód UT WoS článku

001638352500001

EID výsledku v databázi Scopus

2-s2.0-105025676292