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Evaluation of methylation epigenetics changes in micropropagated hop plants

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F14864347%3A_____%2F21%3AN0000016" target="_blank" >RIV/14864347:_____/21:N0000016 - isvavai.cz</a>

  • Výsledek na webu

    <a href="https://www.actahort.org/books/1328/1328_2.htm" target="_blank" >https://www.actahort.org/books/1328/1328_2.htm</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.17660/ActaHortic.2021.1328.2" target="_blank" >10.17660/ActaHortic.2021.1328.2</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Evaluation of methylation epigenetics changes in micropropagated hop plants

  • Popis výsledku v původním jazyce

    In vitro tissue cultures have been used for propagation of plants, due to their totipotency and multiplication efficacy, for more than 50 years. In vitro tissue cultures have also been used for initiation and formation of somaclonal variability by genetic and epigenetic changes caused by development and stress conditions. In vitro tissue culture procedures from meristems have been routinely used to produce hop (Humulus lupulus L.) virus-free rootstocks in the Czech Republic. In this study we investigated the genetic and epigenetic variability of in vitro tissue cultures by molecular methods. We assessed DNA methylation levels and variability of Saaz Osvald’s clones, ‘Sládek’ and ‘Premiant’ cultivars by methylation-sensitive amplification polymorphism (MSAP). In vitro plants, regenerated rootstocks, regenerated and control plants in field conditions were used for analyses. A total 346 clear and repetitive amplified products were detected in MSAP analyses within studied hop plants. We found 16 polymorphic products (4.6% of products) and 64 products with methylation changes (18.5% of products) in the analyses. Demethylation events were 14.5 times more often found than de novo methylation events. The most demethylation changes were found in in vitro plants but only few of them were found in same mericlones under field conditions. A hierarchical cluster analysis was used for the evaluation of molecular genetic variability within individual samples. The resulting dendrogram showed that individual samples of the same cultivar were clustered together. Because of detected demethylation changes for control plants within all studied cultivars, we suggest that certain degree of demethylation occurs in all plants thus it is a natural tool of plant epigenetics and evolution. Even though, micropropagation increased methylation epigenetics, we did not find any phenotypical variation within plants under field conditions.

  • Název v anglickém jazyce

    Evaluation of methylation epigenetics changes in micropropagated hop plants

  • Popis výsledku anglicky

    In vitro tissue cultures have been used for propagation of plants, due to their totipotency and multiplication efficacy, for more than 50 years. In vitro tissue cultures have also been used for initiation and formation of somaclonal variability by genetic and epigenetic changes caused by development and stress conditions. In vitro tissue culture procedures from meristems have been routinely used to produce hop (Humulus lupulus L.) virus-free rootstocks in the Czech Republic. In this study we investigated the genetic and epigenetic variability of in vitro tissue cultures by molecular methods. We assessed DNA methylation levels and variability of Saaz Osvald’s clones, ‘Sládek’ and ‘Premiant’ cultivars by methylation-sensitive amplification polymorphism (MSAP). In vitro plants, regenerated rootstocks, regenerated and control plants in field conditions were used for analyses. A total 346 clear and repetitive amplified products were detected in MSAP analyses within studied hop plants. We found 16 polymorphic products (4.6% of products) and 64 products with methylation changes (18.5% of products) in the analyses. Demethylation events were 14.5 times more often found than de novo methylation events. The most demethylation changes were found in in vitro plants but only few of them were found in same mericlones under field conditions. A hierarchical cluster analysis was used for the evaluation of molecular genetic variability within individual samples. The resulting dendrogram showed that individual samples of the same cultivar were clustered together. Because of detected demethylation changes for control plants within all studied cultivars, we suggest that certain degree of demethylation occurs in all plants thus it is a natural tool of plant epigenetics and evolution. Even though, micropropagation increased methylation epigenetics, we did not find any phenotypical variation within plants under field conditions.

Klasifikace

  • Druh

    D - Stať ve sborníku

  • CEP obor

  • OECD FORD obor

    40401 - Agricultural biotechnology and food biotechnology

Návaznosti výsledku

  • Projekt

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2021

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název statě ve sborníku

    Acta Horticulturae 1328

  • ISBN

    978-94-62613-25-6

  • ISSN

    0567-7572

  • e-ISSN

    2406-6168

  • Počet stran výsledku

    6

  • Strana od-do

    17-22

  • Název nakladatele

    International Society for Horticultural Science

  • Místo vydání

    Leuven

  • Místo konání akce

    Stuttgart

  • Datum konání akce

    8. 3. 2021

  • Typ akce podle státní příslušnosti

    WRD - Celosvětová akce

  • Kód UT WoS článku