Evaluation of methylation epigenetics changes in micropropagated hop plants
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F14864347%3A_____%2F21%3AN0000016" target="_blank" >RIV/14864347:_____/21:N0000016 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.actahort.org/books/1328/1328_2.htm" target="_blank" >https://www.actahort.org/books/1328/1328_2.htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17660/ActaHortic.2021.1328.2" target="_blank" >10.17660/ActaHortic.2021.1328.2</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Evaluation of methylation epigenetics changes in micropropagated hop plants
Popis výsledku v původním jazyce
In vitro tissue cultures have been used for propagation of plants, due to their totipotency and multiplication efficacy, for more than 50 years. In vitro tissue cultures have also been used for initiation and formation of somaclonal variability by genetic and epigenetic changes caused by development and stress conditions. In vitro tissue culture procedures from meristems have been routinely used to produce hop (Humulus lupulus L.) virus-free rootstocks in the Czech Republic. In this study we investigated the genetic and epigenetic variability of in vitro tissue cultures by molecular methods. We assessed DNA methylation levels and variability of Saaz Osvald’s clones, ‘Sládek’ and ‘Premiant’ cultivars by methylation-sensitive amplification polymorphism (MSAP). In vitro plants, regenerated rootstocks, regenerated and control plants in field conditions were used for analyses. A total 346 clear and repetitive amplified products were detected in MSAP analyses within studied hop plants. We found 16 polymorphic products (4.6% of products) and 64 products with methylation changes (18.5% of products) in the analyses. Demethylation events were 14.5 times more often found than de novo methylation events. The most demethylation changes were found in in vitro plants but only few of them were found in same mericlones under field conditions. A hierarchical cluster analysis was used for the evaluation of molecular genetic variability within individual samples. The resulting dendrogram showed that individual samples of the same cultivar were clustered together. Because of detected demethylation changes for control plants within all studied cultivars, we suggest that certain degree of demethylation occurs in all plants thus it is a natural tool of plant epigenetics and evolution. Even though, micropropagation increased methylation epigenetics, we did not find any phenotypical variation within plants under field conditions.
Název v anglickém jazyce
Evaluation of methylation epigenetics changes in micropropagated hop plants
Popis výsledku anglicky
In vitro tissue cultures have been used for propagation of plants, due to their totipotency and multiplication efficacy, for more than 50 years. In vitro tissue cultures have also been used for initiation and formation of somaclonal variability by genetic and epigenetic changes caused by development and stress conditions. In vitro tissue culture procedures from meristems have been routinely used to produce hop (Humulus lupulus L.) virus-free rootstocks in the Czech Republic. In this study we investigated the genetic and epigenetic variability of in vitro tissue cultures by molecular methods. We assessed DNA methylation levels and variability of Saaz Osvald’s clones, ‘Sládek’ and ‘Premiant’ cultivars by methylation-sensitive amplification polymorphism (MSAP). In vitro plants, regenerated rootstocks, regenerated and control plants in field conditions were used for analyses. A total 346 clear and repetitive amplified products were detected in MSAP analyses within studied hop plants. We found 16 polymorphic products (4.6% of products) and 64 products with methylation changes (18.5% of products) in the analyses. Demethylation events were 14.5 times more often found than de novo methylation events. The most demethylation changes were found in in vitro plants but only few of them were found in same mericlones under field conditions. A hierarchical cluster analysis was used for the evaluation of molecular genetic variability within individual samples. The resulting dendrogram showed that individual samples of the same cultivar were clustered together. Because of detected demethylation changes for control plants within all studied cultivars, we suggest that certain degree of demethylation occurs in all plants thus it is a natural tool of plant epigenetics and evolution. Even though, micropropagation increased methylation epigenetics, we did not find any phenotypical variation within plants under field conditions.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
—
OECD FORD obor
40401 - Agricultural biotechnology and food biotechnology
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
Acta Horticulturae 1328
ISBN
978-94-62613-25-6
ISSN
0567-7572
e-ISSN
2406-6168
Počet stran výsledku
6
Strana od-do
17-22
Název nakladatele
International Society for Horticultural Science
Místo vydání
Leuven
Místo konání akce
Stuttgart
Datum konání akce
8. 3. 2021
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
—