Evaluation of the AFLP and MSAP methods as tools for studying of DNA changes in grapevine plants long period after their in vitro thermotherapy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F12%3A00198664" target="_blank" >RIV/62156489:43510/12:00198664 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of the AFLP and MSAP methods as tools for studying of DNA changes in grapevine plants long period after their in vitro thermotherapy

Popis výsledku v původním jazyce

To evaluate the impact of the stress induced by thermotherapy on the primary structure of DNA, AFLP (Amplified Fragment Length Polymorphism) was applied in this study. Epigenetic changes were determined by means of MSAP (Methylation Sensitive Amplified Polymorphism). Studies were carried out on plants at different times after thermotherapy treatments (6 weeks to 5 years after it). The results show nearly identical AFLP-derived fingerprints in all analysed samples from plants treated by in vitro thermotherapy, suggesting that the stress condition did not provoke changes at the sequence level or the method is not suitable to detect possible changes. In contrast, MSAP allowed the observation of differences in epigenetic fingerprints in thermotherapy treated plants. Furthermore, the method allowed to determine the impact in a time dependent and quantifiable fashion. Plants sampled 6 weeks after thermotherapy showed most different epigenetic profiles, whereas plants with longer period after

Název v anglickém jazyce

Evaluation of the AFLP and MSAP methods as tools for studying of DNA changes in grapevine plants long period after their in vitro thermotherapy

Popis výsledku anglicky

To evaluate the impact of the stress induced by thermotherapy on the primary structure of DNA, AFLP (Amplified Fragment Length Polymorphism) was applied in this study. Epigenetic changes were determined by means of MSAP (Methylation Sensitive Amplified Polymorphism). Studies were carried out on plants at different times after thermotherapy treatments (6 weeks to 5 years after it). The results show nearly identical AFLP-derived fingerprints in all analysed samples from plants treated by in vitro thermotherapy, suggesting that the stress condition did not provoke changes at the sequence level or the method is not suitable to detect possible changes. In contrast, MSAP allowed the observation of differences in epigenetic fingerprints in thermotherapy treated plants. Furthermore, the method allowed to determine the impact in a time dependent and quantifiable fashion. Plants sampled 6 weeks after thermotherapy showed most different epigenetic profiles, whereas plants with longer period after

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QH91153" target="_blank" >QH91153: Využití in vitro kultur k ozdravení odrůd ovocných dřevin a révy vinné od virů, fytoplazem a karanténních patogenů pro systém certifikace výsadbového materiálu včetně ověřování kvality.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Horticulturae

ISSN

0567-7572

e-ISSN

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Svazek periodika

961

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

BE - Belgické království

Počet stran výsledku

8

Strana od-do

73-80

Kód UT WoS článku

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EID výsledku v databázi Scopus

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