AFLP-derived methods as a tool for study of genomic, epigenomic and transcriptomic changes in stressed grapevine plants

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F09%3A00152163" target="_blank" >RIV/62156489:43510/09:00152163 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

AFLP-derived methods as a tool for study of genomic, epigenomic and transcriptomic changes in stressed grapevine plants

Popis výsledku v původním jazyce

Many studies describing phenotypic and genetic variation among standard and stressed plants were already published. A possibility to estimate stress-induced changes in genome, epigenome and transcriptome arose by establishing three AFLP-based methods within our laboratory (standard AFLP, methylation-sensitive AFLP and cDNA-AFLP). Three variants of grapevine plants after different stress actions (stress by in vitro cultivation, in vitro thermotherapy and virus infection) were prepared for this study. Incase of standard AFLP application, comparison of obtained spectra via 0/1 binary matrix is usually performed. Within our group of variants the results show insignificant changes in its genomes. MS-AFLP spectra evaluation needs more detailed analysis of results to extract more precise information about DNA methylation characters and impact of individual stress factors on epigenetic changes. From the point of view of functional methodology, the cDNA-AFLP appeared as the most difficult to e

Název v anglickém jazyce

AFLP-derived methods as a tool for study of genomic, epigenomic and transcriptomic changes in stressed grapevine plants

Popis výsledku anglicky

Many studies describing phenotypic and genetic variation among standard and stressed plants were already published. A possibility to estimate stress-induced changes in genome, epigenome and transcriptome arose by establishing three AFLP-based methods within our laboratory (standard AFLP, methylation-sensitive AFLP and cDNA-AFLP). Three variants of grapevine plants after different stress actions (stress by in vitro cultivation, in vitro thermotherapy and virus infection) were prepared for this study. Incase of standard AFLP application, comparison of obtained spectra via 0/1 binary matrix is usually performed. Within our group of variants the results show insignificant changes in its genomes. MS-AFLP spectra evaluation needs more detailed analysis of results to extract more precise information about DNA methylation characters and impact of individual stress factors on epigenetic changes. From the point of view of functional methodology, the cDNA-AFLP appeared as the most difficult to e

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/OC%20116" target="_blank" >OC 116: VLIV STRESU INDUKOVANÉHO PŘI MANIPULACÍCH IN VITRO A TERMOTERAPII NA GENETICKÉ A EPIGENETICKÉ ZMĚNY GENOMU RÉVY VINNÉ</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae 839

ISBN

978-90-6605-612-1

ISSN

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e-ISSN

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Počet stran výsledku

9

Strana od-do

575-583

Název nakladatele

International Society for Horticultural Science

Místo vydání

Leuven

Místo konání akce

Dresden

Datum konání akce

1. 9. 2008

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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