Molecular genetic analysis of Pseudoperonospora humuli populations in hop growing areas in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F14864347%3A_____%2F21%3AN0000018" target="_blank" >RIV/14864347:_____/21:N0000018 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.actahort.org/books/1328/1328_7.htm" target="_blank" >https://www.actahort.org/books/1328/1328_7.htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2021.1328.7" target="_blank" >10.17660/ActaHortic.2021.1328.7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Molecular genetic analysis of Pseudoperonospora humuli populations in hop growing areas in the Czech Republic

Popis výsledku v původním jazyce

Hop downy mildew Pseudoperonospora humuli is a major fungal hop pathogen of worldwide importance, causing severe yield and quality losses. Previous studies of P. humuli in hop growing areas of the United States showed only little genetic variability within the fungal population. The character of Czech P. humuli populations was unknown prior to this work, therefore, we studied the genomic and population diversity by molecular methods. Isolates of P. humuli have been collected during spring starting from commercial fields in the ‘Saaz’ hop growing region over several seasons. Basal spikes from all Czech hop growing areas were collected in 2018 mainly from susceptible cultivars. We analyzed sequential variability of internal transcribed spacer of nuclear ribosomal DNA (ITS) region, β-tubulin gene(β-tub), and the cytochrome c oxidase (cox) cluster (cox2, cox2-cox1 spacer and cox1). Substitution frequencies varied from 0.75% for nuclear ITS regions to 3.14% mitochondrial cox cluster sequences. Phylogenetic analyses showed there was only little sequence variability within Czech P. humuli isolates across studied population localities and years. We also used 25 simple sequence repeats (SSR) markers for the evaluation of genetic variation. Only ten primer combinations amplified SSR markers and all amplified SSR markers were monomorphic. Therefore, genetic structure of Czech P. humuli populations differed little, and it can be suggested that P. humuli populations have clonal character in the Czech Republic.

Název v anglickém jazyce

Molecular genetic analysis of Pseudoperonospora humuli populations in hop growing areas in the Czech Republic

Popis výsledku anglicky

Hop downy mildew Pseudoperonospora humuli is a major fungal hop pathogen of worldwide importance, causing severe yield and quality losses. Previous studies of P. humuli in hop growing areas of the United States showed only little genetic variability within the fungal population. The character of Czech P. humuli populations was unknown prior to this work, therefore, we studied the genomic and population diversity by molecular methods. Isolates of P. humuli have been collected during spring starting from commercial fields in the ‘Saaz’ hop growing region over several seasons. Basal spikes from all Czech hop growing areas were collected in 2018 mainly from susceptible cultivars. We analyzed sequential variability of internal transcribed spacer of nuclear ribosomal DNA (ITS) region, β-tubulin gene(β-tub), and the cytochrome c oxidase (cox) cluster (cox2, cox2-cox1 spacer and cox1). Substitution frequencies varied from 0.75% for nuclear ITS regions to 3.14% mitochondrial cox cluster sequences. Phylogenetic analyses showed there was only little sequence variability within Czech P. humuli isolates across studied population localities and years. We also used 25 simple sequence repeats (SSR) markers for the evaluation of genetic variation. Only ten primer combinations amplified SSR markers and all amplified SSR markers were monomorphic. Therefore, genetic structure of Czech P. humuli populations differed little, and it can be suggested that P. humuli populations have clonal character in the Czech Republic.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae 1328

ISBN

978-94-62613-25-6

ISSN

0567-7572

e-ISSN

2406-6168

Počet stran výsledku

8

Strana od-do

55-62

Název nakladatele

International Society for Horticultural Science

Místo vydání

Leuven

Místo konání akce

Stuttgart

Datum konání akce

8. 3. 2021

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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