How to study common hop (Humulus lupulus L.) karyotype evolution: genomic and cytogenetics approaches

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F14864347%3A_____%2F23%3AN0000076" target="_blank" >RIV/14864347:_____/23:N0000076 - isvavai.cz</a>

Výsledek na webu

<a href="https://plan.core-apps.com/pag_2023/abstract/7d686e5d4c7cd9f48449347cbcd380e9" target="_blank" >https://plan.core-apps.com/pag_2023/abstract/7d686e5d4c7cd9f48449347cbcd380e9</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

How to study common hop (Humulus lupulus L.) karyotype evolution: genomic and cytogenetics approaches

Popis výsledku v původním jazyce

HORÁKOVÁ, Lucie, Václav BAČOVSKÝ, Radim ČEGAN, Bohuslav JANOUŠEK, Marcel HUBINSKÝ, Josef PATZAK and Roman HOBZA. How to study common hop (Humulus lupulus L.) karyotype evolution: genomic and cytogenetics approaches [poster]. Plant and Animal Genome XXX Conference, San Diego, USA, January 13-18, 2023. Humulus lupulus L. (common hop.), a member of the Cannabaceae family, is a dioecious plant that possesses heteromorphic sex chromosomes (20, XX in females and 20, XY in males) and has X:A sex determination system. Despite recent advances in the field of genome organization in other plant models (Arabidopsis, Oryza, etc.), evolutionary processes shaping genomic landscape in hop are still poorly understood. Here, we took an advantage of the next-generation sequencing technology and bioinformatics to compare H. lupulus and H. japonicus (Japanese hop) genomes. We found various level of DNA repeats abundance between these two closely related species, and newly identified cytogenetic markers to study individual chromosomes and chromosomal rearrangements. We localized the position of several candidates on metaphase chromosomes using fluorescence in situ hybridization (FISH), and screened sequence homology for shared satellites. Comparative analysis of hop's genomes suggested extensive chromosomal rearrangements during their karyotype and sex chromosome evolution. Although both species differ in chromosome number and sex chromosome system, major satellites units have higher similarities and surprisingly the same pattern on metaphase chromosomes. Our findings provide new insight into the evolutionary processes of the Cannabaceae family and may help to decipher the origin of sex chromosome in common hop cultivars.

Název v anglickém jazyce

How to study common hop (Humulus lupulus L.) karyotype evolution: genomic and cytogenetics approaches

Popis výsledku anglicky

HORÁKOVÁ, Lucie, Václav BAČOVSKÝ, Radim ČEGAN, Bohuslav JANOUŠEK, Marcel HUBINSKÝ, Josef PATZAK and Roman HOBZA. How to study common hop (Humulus lupulus L.) karyotype evolution: genomic and cytogenetics approaches [poster]. Plant and Animal Genome XXX Conference, San Diego, USA, January 13-18, 2023. Humulus lupulus L. (common hop.), a member of the Cannabaceae family, is a dioecious plant that possesses heteromorphic sex chromosomes (20, XX in females and 20, XY in males) and has X:A sex determination system. Despite recent advances in the field of genome organization in other plant models (Arabidopsis, Oryza, etc.), evolutionary processes shaping genomic landscape in hop are still poorly understood. Here, we took an advantage of the next-generation sequencing technology and bioinformatics to compare H. lupulus and H. japonicus (Japanese hop) genomes. We found various level of DNA repeats abundance between these two closely related species, and newly identified cytogenetic markers to study individual chromosomes and chromosomal rearrangements. We localized the position of several candidates on metaphase chromosomes using fluorescence in situ hybridization (FISH), and screened sequence homology for shared satellites. Comparative analysis of hop's genomes suggested extensive chromosomal rearrangements during their karyotype and sex chromosome evolution. Although both species differ in chromosome number and sex chromosome system, major satellites units have higher similarities and surprisingly the same pattern on metaphase chromosomes. Our findings provide new insight into the evolutionary processes of the Cannabaceae family and may help to decipher the origin of sex chromosome in common hop cultivars.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/GA22-00301S" target="_blank" >GA22-00301S: Dynamika genomů v kontextu evoluce pohlavních chromozomů u rodu Humulus</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů