Dynamic patterns of repeats and retrotransposons in the centromeres of Humulus lupulus L.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00638532" target="_blank" >RIV/61389030:_____/25:00638532 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/25:00638532 RIV/00216224:14310/25:00141846 RIV/14864347:_____/25:N0000007 RIV/14864347:_____/25:N0000008

Výsledek na webu

<a href="https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.70380" target="_blank" >https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.70380</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/nph.70380" target="_blank" >10.1111/nph.70380</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dynamic patterns of repeats and retrotransposons in the centromeres of Humulus lupulus L.

Popis výsledku v původním jazyce

The centromere has a conserved function across eukaryotes, however, the associated DNA sequences exhibit remarkable diversity in both size and structure. In plants, some species possess well-defined centromeres dominated by tandem satellite repeats and centromeric retrotransposons, while others have centromeric regions composed almost entirely of retrotransposons. Using a combination of bioinformatic, molecular, and cytogenetic approaches, we analyzed the centromeric landscape of Humulus lupulus. We identified novel centromeric repeats and characterized two types of centromeric organization. Cytogenetic localization on metaphase chromosomes confirmed the genomic distribution of the major repeats and revealed unique centromeric organization specifically on chromosomes 2, 8, and Y. Two centromeric types are composed of the major repeats SaazCEN and SaazCRM1 (Ty3/Gypsy) which are further accompanied by chromosome-specific centromeric satellites, Saaz40, Saaz293, Saaz85, and HuluTR120. Chromosome 2 displays unbalanced segregation during mitosis and meiosis, implicating an important role for its centromere structure in segregation patterns. Moreover, chromosome 2-specific centromeric repeat Saaz293 is a new marker for studying aneuploidy in hops. Our findings provide new insights into chromosome segregation in hops and highlight the diversity and complexity of the centromere organization in H. lupulus.

Název v anglickém jazyce

Dynamic patterns of repeats and retrotransposons in the centromeres of Humulus lupulus L.

Popis výsledku anglicky

The centromere has a conserved function across eukaryotes, however, the associated DNA sequences exhibit remarkable diversity in both size and structure. In plants, some species possess well-defined centromeres dominated by tandem satellite repeats and centromeric retrotransposons, while others have centromeric regions composed almost entirely of retrotransposons. Using a combination of bioinformatic, molecular, and cytogenetic approaches, we analyzed the centromeric landscape of Humulus lupulus. We identified novel centromeric repeats and characterized two types of centromeric organization. Cytogenetic localization on metaphase chromosomes confirmed the genomic distribution of the major repeats and revealed unique centromeric organization specifically on chromosomes 2, 8, and Y. Two centromeric types are composed of the major repeats SaazCEN and SaazCRM1 (Ty3/Gypsy) which are further accompanied by chromosome-specific centromeric satellites, Saaz40, Saaz293, Saaz85, and HuluTR120. Chromosome 2 displays unbalanced segregation during mitosis and meiosis, implicating an important role for its centromere structure in segregation patterns. Moreover, chromosome 2-specific centromeric repeat Saaz293 is a new marker for studying aneuploidy in hops. Our findings provide new insights into chromosome segregation in hops and highlight the diversity and complexity of the centromere organization in H. lupulus.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

New Phytologist

ISSN

0028-646X

e-ISSN

1469-8137

Svazek periodika

247

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

15

Strana od-do

2766-2780

Kód UT WoS článku

001531851200001

EID výsledku v databázi Scopus

2-s2.0-105010870504