Identification of freshwater zooplankton species using protein profiling and principal component analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25173154%3A_____%2F18%3AN0000007" target="_blank" >RIV/25173154:_____/18:N0000007 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22320/18:43915515 RIV/60461373:22330/18:43915515 RIV/60461373:22340/18:43915515

Výsledek na webu

<a href="https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lom3.10238" target="_blank" >https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lom3.10238</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/lom3.10238" target="_blank" >10.1002/lom3.10238</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of freshwater zooplankton species using protein profiling and principal component analysis

Popis výsledku v původním jazyce

Crustaceans are vital components of the freshwater zooplankton that link various trophic levels. Because crustaceans, particularly cladocerans of the Daphnia genus, are sensitive to environmental changes, they can serve as indicators of environmental fluctuations. Therefore, it is highly desirable to have a fast and reliable method for the identification of individual Daphnia species. In this study, we demonstrated the ability of protein profiling to distinguish between freshwater zooplankton species. Individual specimens were morphologically identified before being analyzed by proteomic fingerprinting using matrix‐assisted laser desorption/ionization time of flight mass spectrometry (MALDI‐TOF MS). The obtained mass spectra were used to create a reference library. Subsequently, the individual species were successfully identified using Biotyper software. The variability between the spectra of individual Daphnia species was verified by principal component analysis (PCA). Our results suggest that the combination of proteome fingerprinting using MALDI‐TOF MS and PCA has considerable potential as a rapid tool for the unambiguous identification of individual species of freshwater zooplankton without the need for expert morphological analysis.

Název v anglickém jazyce

Identification of freshwater zooplankton species using protein profiling and principal component analysis

Popis výsledku anglicky

Crustaceans are vital components of the freshwater zooplankton that link various trophic levels. Because crustaceans, particularly cladocerans of the Daphnia genus, are sensitive to environmental changes, they can serve as indicators of environmental fluctuations. Therefore, it is highly desirable to have a fast and reliable method for the identification of individual Daphnia species. In this study, we demonstrated the ability of protein profiling to distinguish between freshwater zooplankton species. Individual specimens were morphologically identified before being analyzed by proteomic fingerprinting using matrix‐assisted laser desorption/ionization time of flight mass spectrometry (MALDI‐TOF MS). The obtained mass spectra were used to create a reference library. Subsequently, the individual species were successfully identified using Biotyper software. The variability between the spectra of individual Daphnia species was verified by principal component analysis (PCA). Our results suggest that the combination of proteome fingerprinting using MALDI‐TOF MS and PCA has considerable potential as a rapid tool for the unambiguous identification of individual species of freshwater zooplankton without the need for expert morphological analysis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10503 - Water resources

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Limnology and Oceanography: Methods

ISSN

1541-5856

e-ISSN

—

Svazek periodika

16

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

199-204

Kód UT WoS článku

000428449500004

EID výsledku v databázi Scopus

2-s2.0-85041180794&