In Vitro Propagation of the P-HL Dwarfing Sweet Cherry Rootstocks

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

In Vitro Propagation of the P-HL Dwarfing Sweet Cherry Rootstocks

Popis výsledku v původním jazyce

We have developed a rapid shoot multiplication procedure for in vitro propagation of dwarfing P-HL rootstocks (P-HL-A, P-HL-B, P-HL-C) for sweet cherries selected in RBIP Holovousy. Shoot tips were successfully established in vitro and micropropagated onMurashige and Skoog (MS) based media containing different concentration of 6-benzylaminopurine (BAP). Multiplication rates varied depending on the rootstock and concentration of BAP. The highest multiplication rate was obtained for P-HL-A rootstock thatproduced 10.9 +-0.5 shoots (longer than 10 mm) on MS medium containing 1.5 mg/L BAP. The lowest multiplication rate was obtained for P-HL-B producing only 2.0 +- 0.3 shoots on MS medium containing 0.2 mg/L BAP. Higher concentration of BAP 2 mg/L was deleterious to shoot growth and multiplication rate. Effects of antibiotic cefotaxime at 200 mg/L on eliminating bacterial contamination were evaluated during in vitro multiplication. Also reported is the in vitro rooting.

Název v anglickém jazyce

In Vitro Propagation of the P-HL Dwarfing Sweet Cherry Rootstocks

Popis výsledku anglicky

We have developed a rapid shoot multiplication procedure for in vitro propagation of dwarfing P-HL rootstocks (P-HL-A, P-HL-B, P-HL-C) for sweet cherries selected in RBIP Holovousy. Shoot tips were successfully established in vitro and micropropagated onMurashige and Skoog (MS) based media containing different concentration of 6-benzylaminopurine (BAP). Multiplication rates varied depending on the rootstock and concentration of BAP. The highest multiplication rate was obtained for P-HL-A rootstock thatproduced 10.9 +-0.5 shoots (longer than 10 mm) on MS medium containing 1.5 mg/L BAP. The lowest multiplication rate was obtained for P-HL-B producing only 2.0 +- 0.3 shoots on MS medium containing 0.2 mg/L BAP. Higher concentration of BAP 2 mg/L was deleterious to shoot growth and multiplication rate. Effects of antibiotic cefotaxime at 200 mg/L on eliminating bacterial contamination were evaluated during in vitro multiplication. Also reported is the in vitro rooting.

Druh

D - Stať ve sborníku

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

978-90-6605-551-3

ISSN

0567-7572

e-ISSN

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Počet stran výsledku

5

Strana od-do

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Název nakladatele

International Society Horticultural Science

Místo vydání

Leuven

Místo konání akce

Bursa

Datum konání akce

6. 6. 2005

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000260482900059