In vitro establishment and proliferation of apple cultivars

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F16%3AN0000010" target="_blank" >RIV/25271121:_____/16:N0000010 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17660/ActaHortic.2016.1113.15" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2016.1113.15</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2016.1113.15" target="_blank" >10.17660/ActaHortic.2016.1113.15</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro establishment and proliferation of apple cultivars

Popis výsledku v původním jazyce

The goal of this study was to investigate in vitro multiplication protocols for the use with apple cultivars 'Bohemia, 'Rubimeg' and 'Primadela' grown in the Czech Republic. For the initiation phase, dormant shoots (15-20 cm in length) were collected from field germplasm at Research and Breeding of Pomology Holovousy Ltd. Shoot tips (5 to 10 mm in length) were successfully established in vitro using mercuric chloride at a concentration of 0.15% as a sterilization solution. To assess the effect of plant growth regulators on multiplication, in vitro shoots were cultured on the basal MS medium supplemented with 1, 2 and 4 mg L-1 6-benzylaminopurine (BAP), 0.5 and 1 mg L-1 thidiazuron (TDZ), or 1, 2 and 4 mg L-1 kinetin. BAP at the highest concentrations (2 and 4 mg L-1) and TDZ were found to be more effective than kinetin for shoot multiplication. Generally, the highest proliferation rate was obtained for 'Bohemia' that produced 4.1 shoots (longer than 10 mm) on medium containing 4 mg L-1 BAP. On the contrary, the lowest proliferation rate (1.0) was obtained for 'Rubimeg' and 'Primadela', which almost did not multiply on MS medium containing the lowest concentration of BAP (1 mg L-1). Kinetin at the tested concentrations did not promote satisfactorily proliferation of the three apple cultivars (proliferation between 1.1 and 1.3). Efficient micropropagation protocols are a prerequisite for utilization of sophisticated in vitro virus elimination techniques (in vitro thermotherapy and chemotherapy).

Název v anglickém jazyce

In vitro establishment and proliferation of apple cultivars

Popis výsledku anglicky

The goal of this study was to investigate in vitro multiplication protocols for the use with apple cultivars 'Bohemia, 'Rubimeg' and 'Primadela' grown in the Czech Republic. For the initiation phase, dormant shoots (15-20 cm in length) were collected from field germplasm at Research and Breeding of Pomology Holovousy Ltd. Shoot tips (5 to 10 mm in length) were successfully established in vitro using mercuric chloride at a concentration of 0.15% as a sterilization solution. To assess the effect of plant growth regulators on multiplication, in vitro shoots were cultured on the basal MS medium supplemented with 1, 2 and 4 mg L-1 6-benzylaminopurine (BAP), 0.5 and 1 mg L-1 thidiazuron (TDZ), or 1, 2 and 4 mg L-1 kinetin. BAP at the highest concentrations (2 and 4 mg L-1) and TDZ were found to be more effective than kinetin for shoot multiplication. Generally, the highest proliferation rate was obtained for 'Bohemia' that produced 4.1 shoots (longer than 10 mm) on medium containing 4 mg L-1 BAP. On the contrary, the lowest proliferation rate (1.0) was obtained for 'Rubimeg' and 'Primadela', which almost did not multiply on MS medium containing the lowest concentration of BAP (1 mg L-1). Kinetin at the tested concentrations did not promote satisfactorily proliferation of the three apple cultivars (proliferation between 1.1 and 1.3). Efficient micropropagation protocols are a prerequisite for utilization of sophisticated in vitro virus elimination techniques (in vitro thermotherapy and chemotherapy).

Druh

D - Stať ve sborníku

CEP obor

GC - Pěstování rostlin, osevní postupy

OECD FORD obor

—

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings of the International Symposium on Micropropagation and In Vitro Techniques

ISBN

978-94-62611-07-8

ISSN

0567-7572

e-ISSN

—

Počet stran výsledku

5

Strana od-do

107-111

Název nakladatele

International Society for Horticultural Science

Místo vydání

Leuven, Belgium

Místo konání akce

Brisbane, Australia

Datum konání akce

1. 1. 2014

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000391238600015