Protocol for rapid in vitro shoot multiplication of ancient apple cultivars and landraces, grown in Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F25271121%3A_____%2F19%3AN0000099" target="_blank" >RIV/25271121:_____/19:N0000099 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1242.102" target="_blank" >http://dx.doi.org/10.17660/ActaHortic.2019.1242.102</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17660/ActaHortic.2019.1242.102" target="_blank" >10.17660/ActaHortic.2019.1242.102</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Protocol for rapid in vitro shoot multiplication of ancient apple cultivars and landraces, grown in Czech Republic

Popis výsledku v původním jazyce

The apple fruit is the most economically important fruit species in the temperate climatic zone of central Europe. In 2012, a project was initiated to test the resistance of apple landraces and older cultivars to fire blight. The aim of this study was to develop a protocol for rapid in vitro shoot multiplication of selected apple cultivars. Successful in vitro propagation, which can be carried out all year round, could provide an alternative method to produce stock material of particular cultivars for experiments with artificial inoculation in safe and controllable laboratory conditions. Selected genotypes were successfully established in vitro using 0.15% mercuric chloride as disinfectant solution. To determine favourable conditions for shoot initiation and development, six proliferation MS media containing 1, 2 and 4 mg L‑1 BAP (6-benzylaminopurine), 0.5 and 1 mg L‑1 TDZ (thidiazuron) or 10 mg L‑1 2iP (6-(γ,γ-dimethylallylamino) purine) were tested. All apple genotypes in this study responded favourably to micropropagation in MS medium. The multiplication rate varied depending on the cultivar and concentration of cytokinins between 1.1 and 7.1. The highest proliferation rate was obtained for apple cultivar ‘Hvezdnata reneta’ which produced 7.1 in vitro shoots (longer than 10 mm) on MS medium containing 1 mg L‑1 TDZ. On the contrary, the lowest proliferation rate 1.1 in our experiments was obtained for cultivar ‘Kminova reneta’ on MS medium with 10 mg L‑1 2iP. Our results confirmed the preliminary findings which indicated that TDZ was an important plant growth regulator for proliferation and growth in apple micropropagation. However, some abnormalities such as swollen shoots shorter than 10 mm with narrow leaves were observed during shoot multiplication on MS media with TDZ. The cytokinin BAP gave satisfactory results for multiplication in a concentration of 4 mg L‑1. All cultivars had well-developed leaves on media containing BAP.

Název v anglickém jazyce

Protocol for rapid in vitro shoot multiplication of ancient apple cultivars and landraces, grown in Czech Republic

Popis výsledku anglicky

The apple fruit is the most economically important fruit species in the temperate climatic zone of central Europe. In 2012, a project was initiated to test the resistance of apple landraces and older cultivars to fire blight. The aim of this study was to develop a protocol for rapid in vitro shoot multiplication of selected apple cultivars. Successful in vitro propagation, which can be carried out all year round, could provide an alternative method to produce stock material of particular cultivars for experiments with artificial inoculation in safe and controllable laboratory conditions. Selected genotypes were successfully established in vitro using 0.15% mercuric chloride as disinfectant solution. To determine favourable conditions for shoot initiation and development, six proliferation MS media containing 1, 2 and 4 mg L‑1 BAP (6-benzylaminopurine), 0.5 and 1 mg L‑1 TDZ (thidiazuron) or 10 mg L‑1 2iP (6-(γ,γ-dimethylallylamino) purine) were tested. All apple genotypes in this study responded favourably to micropropagation in MS medium. The multiplication rate varied depending on the cultivar and concentration of cytokinins between 1.1 and 7.1. The highest proliferation rate was obtained for apple cultivar ‘Hvezdnata reneta’ which produced 7.1 in vitro shoots (longer than 10 mm) on MS medium containing 1 mg L‑1 TDZ. On the contrary, the lowest proliferation rate 1.1 in our experiments was obtained for cultivar ‘Kminova reneta’ on MS medium with 10 mg L‑1 2iP. Our results confirmed the preliminary findings which indicated that TDZ was an important plant growth regulator for proliferation and growth in apple micropropagation. However, some abnormalities such as swollen shoots shorter than 10 mm with narrow leaves were observed during shoot multiplication on MS media with TDZ. The cytokinin BAP gave satisfactory results for multiplication in a concentration of 4 mg L‑1. All cultivars had well-developed leaves on media containing BAP.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

978-94-62612-39-6

ISSN

—

e-ISSN

—

Počet stran výsledku

6

Strana od-do

691-696

Název nakladatele

ISHS

Místo vydání

Leuven

Místo konání akce

Chania, Kréta, Řecko

Datum konání akce

10. 7. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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