Micropropagation of Czech Apple Cultivars

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Micropropagation of Czech Apple Cultivars

Popis výsledku v původním jazyce

The objective of this study was to investigate micropropagation protocols for use with three apple cultivars (James Grieve Compact, Jarka and Mivibe) bred in the Czech Republic. Apple shoot tips (5 to 10 mm in length) were established in vitro using 0.15% HgCl2 as sterilization solution. Contamination rate, survival and development of shoots from excised shoot tips were analyzed after sterilization. For the three cultivars, the effect of three growth regulators 1, 2 and 4 mg L-1 BAP (6- benzylaminopurine), 0.5 and 1 mg L-1 TDZ or 10 mg L-1 2iP in basal MS medium on proliferation, callus formation and shoot morphology is shown. Values of proliferation rate varied between 1.1 and 3.6. Generally, the highest rate was obtained for cultivar Jarka that produced 3.6 new shoots on MS medium containing 4 mg L-1 BAP. The lowest number of newly formed shoots (1.1) was noted for cultivars James Grieve Compact and Mivibe on MS medium with low concentration of BAP (1 mg L-1). In vitro plants of Jame

Název v anglickém jazyce

Micropropagation of Czech Apple Cultivars

Popis výsledku anglicky

The objective of this study was to investigate micropropagation protocols for use with three apple cultivars (James Grieve Compact, Jarka and Mivibe) bred in the Czech Republic. Apple shoot tips (5 to 10 mm in length) were established in vitro using 0.15% HgCl2 as sterilization solution. Contamination rate, survival and development of shoots from excised shoot tips were analyzed after sterilization. For the three cultivars, the effect of three growth regulators 1, 2 and 4 mg L-1 BAP (6- benzylaminopurine), 0.5 and 1 mg L-1 TDZ or 10 mg L-1 2iP in basal MS medium on proliferation, callus formation and shoot morphology is shown. Values of proliferation rate varied between 1.1 and 3.6. Generally, the highest rate was obtained for cultivar Jarka that produced 3.6 new shoots on MS medium containing 4 mg L-1 BAP. The lowest number of newly formed shoots (1.1) was noted for cultivars James Grieve Compact and Mivibe on MS medium with low concentration of BAP (1 mg L-1). In vitro plants of Jame

Druh

D - Stať ve sborníku

CEP obor

GC - Pěstování rostlin, osevní postupy

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Acta Horticulturae

ISBN

978-94-6261-077-4

ISSN

0567-7572

e-ISSN

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Počet stran výsledku

5

Strana od-do

267-271

Název nakladatele

International Society for Horticultural Science

Místo vydání

Leuven, Belgium

Místo konání akce

Coimbra, Portugal

Datum konání akce

2. 6. 2013

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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